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Folia Microbiologica

, Volume 52, Issue 5, pp 451–456 | Cite as

Glucosylglycerate is an osmotic solute and an extracellular metabolite produced byStreptomyces caelestis

  • S. Pospíšil
  • P. Halada
  • M. Petříček
  • P. Sedmera
Article

Abstract

Streptomyces caelestis DSM 40084produces two osmolytes,viz. 2-O-(α-d-glucopyranosyl)-ζ-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2× in response to a step change of medium osmolarity caused by 0.3 % NaCl.1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is aS. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract-(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genusStreptomyces and on its free excretion to the medium.

Keywords

Streptomyces Trehalose Fermentation Broth Compatible Solute Ergot Alkaloid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

1D-TOCSY

one-dimensional total-correlated spectroscopy

CD

chemically defined (medium)

ESI MS

electrospray ionization mass spectrometry

GG

2-O-(α-d-glucopyranosyl)-ξ-glyceric acid

HMBC

heteronuclear multiple bond correlation

MS

mass spectrometry

NMR

NMR spectroscopy

YEA

complex (medium)

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • S. Pospíšil
    • 1
  • P. Halada
    • 1
  • M. Petříček
    • 1
  • P. Sedmera
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia

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