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Folia Microbiologica

, Volume 52, Issue 4, pp 423–427 | Cite as

Biodegradation of chlorphyrifos byKlebsiella sp. isolated from an activated sludge sample of waste water treatment plant in damascus

  • I. GhanemEmail author
  • M. Orfi
  • M. Shamma
Article

Abstract

A chlorpyrifos (CPY)-degrading bacterial strain was isolated from an activated sludge sample collected from theDamascus Wastewater Treatment Plant, Syria. The isolation ofKlebsiella sp. was facilitated by the addition of CPY at a rate of 3.84 g/L of sludge weekly (selection pressure). Identification ofKlebsiella sp. was done using major staining and biochemical differentiation tests (Gram stain, cytochrome oxidase and some relevant saccharide fermentation tests using biochemical assays).Klebsiella sp. was maintained by culturing in a poor medium consisting of mineral salts and CPY as the sole carbon source. When 3 activated sludge samples were incubated in the presence of CPY (13.9 g/L sludge), 46 % of added CPY were degraded within 4 d. By comparison, within 4 d the isolatedKlebsiella sp. was found to break down 92 % of CPY when co-incubated in a poor mineral medium in which CPY was the sole carbon source (13.9 g/L poor medium). IsolatedKlebsiella sp. was able to tolerate up to 17.3 g of CPY in the poor medium.

Keywords

Sole Carbon Source Chlorpyrifos American Public Health Association Methyl Parathion Waste Water Treatment Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CPY

chlorpyrifos [(O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphothioate]

TCP

O-(3,5,6-trichloro-2-pyridyl)phosphothioate

DWTP

Damascus Wastewater Treatment Plant

EC

emulsifiable concentrate

GC

gas chromatography

MSM

mineral salt medium

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  1. 1.Biotechnology DepartmentAtomic Energy Commission of SyriaDamascusSyria

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