Folia Microbiologica

, Volume 52, Issue 4, pp 315–324

Phenotypic and genotypic analysis ofBorrelia spp. isolated fromIxodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction

  • D. Hulínská
  • J. Votýpka
  • B. Kříž
  • N. Holínková
  • J. Nováková
  • V. Hulínský
Article

DOI: 10.1007/BF02932085

Cite this article as:
Hulínská, D., Votýpka, J., Kříž, B. et al. Folia Microbiol (2007) 52: 315. doi:10.1007/BF02932085

Abstract

The genotype ofBorrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks.Borrelia determination was based on partial sequencing of the 16S rRNA gene and real-time polymerase chain reactions for identification and quantitation ofospA andrecA genes. DifferentBorrelia spp. were identified;B. garinii in 40 % ticks followed byB. afzelii (36.3 %),B. burgdorferi sensu stricto (12.9 %),B. valaisiana (3.5 %),B. lusitaniae (0.8 %),B. bissettii (0.5 %) andB. miyamotoi-like (0.5 %). Cultivation of 30 borrelia strains in BSK-H medium, among themB. valaisiana, B. bissettii-like andB. miyamotoi-like strains was unique in Czechia. Calibrated microfluidic-based quantification showed differences in the concentration of the nucleic acids and molar mass of the outer surface proteins of differentBorrelia spp. with standard sensitivity and specificity and was helpful for their identification. The outer surface protein OspA was absent inB. miyamotoi-like and the OspB protein inB. valaisiana, B. lusitaniae and in three subtypes ofB. garinii.

Abbreviations

Bbl

Borrelia burgdorferi sensu lato

BSK

Barbour-Stoenner-Kelly medium

DFM

dark-field microscopy

EE

Experion electrophoretic (chips)

EM

erythema migrans

LB

Lyme borreliosis

Bbs

B. burgdorferi sensu stricto

OspA, OspB, OspC

outer surface proteins A, B, C

PBS

phosphate-buffered saline

qPCR

quantitative polymerase chain reaction

RT-PCR

real-time PCR

SDS

sodium dodecylsulfate

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • D. Hulínská
    • 1
  • J. Votýpka
    • 1
  • B. Kříž
    • 1
  • N. Holínková
    • 1
  • J. Nováková
    • 2
  • V. Hulínský
    • 3
  1. 1.National Reference Laboratory for Lyme Borreliosis, Center of Epidemiology and MicrobiologyNational Institute of Public HealthPragueCzechia
  2. 2.BioRad Ltd.PragueCzechia
  3. 3.Department of Internal MedicineRegional Hospital NymburkNymburkCzechia

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