Folia Microbiologica

, Volume 52, Issue 4, pp 315–324

Phenotypic and genotypic analysis ofBorrelia spp. isolated fromIxodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction

  • D. Hulínská
  • J. Votýpka
  • B. Kříž
  • N. Holínková
  • J. Nováková
  • V. Hulínský

DOI: 10.1007/BF02932085

Cite this article as:
Hulínská, D., Votýpka, J., Kříž, B. et al. Folia Microbiol (2007) 52: 315. doi:10.1007/BF02932085


The genotype ofBorrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks.Borrelia determination was based on partial sequencing of the 16S rRNA gene and real-time polymerase chain reactions for identification and quantitation ofospA andrecA genes. DifferentBorrelia spp. were identified;B. garinii in 40 % ticks followed byB. afzelii (36.3 %),B. burgdorferi sensu stricto (12.9 %),B. valaisiana (3.5 %),B. lusitaniae (0.8 %),B. bissettii (0.5 %) andB. miyamotoi-like (0.5 %). Cultivation of 30 borrelia strains in BSK-H medium, among themB. valaisiana, B. bissettii-like andB. miyamotoi-like strains was unique in Czechia. Calibrated microfluidic-based quantification showed differences in the concentration of the nucleic acids and molar mass of the outer surface proteins of differentBorrelia spp. with standard sensitivity and specificity and was helpful for their identification. The outer surface protein OspA was absent inB. miyamotoi-like and the OspB protein inB. valaisiana, B. lusitaniae and in three subtypes ofB. garinii.



Borrelia burgdorferi sensu lato


Barbour-Stoenner-Kelly medium


dark-field microscopy


Experion electrophoretic (chips)


erythema migrans


Lyme borreliosis


B. burgdorferi sensu stricto

OspA, OspB, OspC

outer surface proteins A, B, C


phosphate-buffered saline


quantitative polymerase chain reaction


real-time PCR


sodium dodecylsulfate

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • D. Hulínská
    • 1
  • J. Votýpka
    • 1
  • B. Kříž
    • 1
  • N. Holínková
    • 1
  • J. Nováková
    • 2
  • V. Hulínský
    • 3
  1. 1.National Reference Laboratory for Lyme Borreliosis, Center of Epidemiology and MicrobiologyNational Institute of Public HealthPragueCzechia
  2. 2.BioRad Ltd.PragueCzechia
  3. 3.Department of Internal MedicineRegional Hospital NymburkNymburkCzechia

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