Effects of morphology and rheology on neofructosyltransferase production byPenicillium citrinum
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In this study, we investigated the relationship between the morphology and the rheological properties ofPenicillium citrinum to improve the production of neo-fructosyltransferase (neo-FTase). In a 2.5 L bioreactor culture ofP. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5 vvm were 8.14 g/L and 53.2×10−3 U/mL, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies,P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation byP. citrinum was dependent on cell growth and fungal morphology.
Keywordsneo-fructosyltransferase neo-fructooligosaccharide pellet morphology Penicillium citrinum rheology
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