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Folia Microbiologica

, Volume 51, Issue 6, pp 573–578 | Cite as

The role of Mn-dependent peroxidase in dye decolorization by static and agitated cultures ofIrpex lacteus

  • K. SvobodováEmail author
  • P. Erbanová
  • J. Sklenář
  • Č. Novotný
Article

Abstract

Dye decolorization capacity of two white-rot fungi,Irpex lacteus andPhanerochaete chrysosporium, was compared in N-limited liquid cultures. The agitated cultures showed lower ability to decolorize azo dyes Reactive Orange 16 and Naphthol Blue Black than static cultures. Similar effect was also observed with other structurally different synthetic dyes. The effect of surfactants on the decolorization process is discussed. A significant increase in the Reactive Orange 16 decolorization by the agitatedI. lacteus cultures was observed after adding 0.1% Tween 80, following a higher Mn-dependent peroxidase production. Thein vitro dye decolorization using the purified enzyme proved its decolorization ability.

Keywords

Nonionic Surfactant Ligninolytic Enzyme Manganese Peroxidase Veratryl Alcohol Ligninolytic Fungus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BPB

Bromophenol Blue

CSB

Chicago Sky Blue

CuP

copper(II) phthalocyanine

DB3

Disperse Blue 3

DMAB

dimethylamine-borane

LiP

lignin peroxidases

MB

Methylene Blue

MBTH

3-methyl-2-benzothiazolinone hydrazone

MnP

Mn-dependent peroxidases

NBB

Naphthol Blue Black

RBBR

Remazol Brilliant Blue R

RO16

Reactive Orange 16

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2006

Authors and Affiliations

  • K. Svobodová
    • 1
    Email author
  • P. Erbanová
    • 1
  • J. Sklenář
    • 1
  • Č. Novotný
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzechia

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