Comparative studies of differential expression of chitinolytic enzymes encoded bychiA, chiB, chiC andnagA genes inAspergillus nidulans
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N-Acetyl-d-glucosamine, chito-oligomers and carbon starvation regulatedchiA, chiB, andnagA gene expressions inAspergillus nidulans cultures. The gene expression patterns of the main extracellular endochitinase ChiB and theN-acetyl-β-d-glucosaminidase NagA were similar, and the ChiB-NagA enzyme system may play a morphological and/or nutritional role during autolysis. Alterations in the levels of reactive oxygen species or in the glutathione-glutathione disulfide redox balance, characteristic physiological changes developing in ageing and autolyzing fungal cultures, did not affect the regulation of either the growth-relatedchiA or the autolysis-coupledchiB genes although both of them were down-regulated under diamide stress. The transcription of thechiC gene with unknown physiological function was repressed by increased intracellular superoxide concentration.
coefficient of variance
dry cell mass
β-N-acetylhexosaminidase (N-acetyl-β-d-glucosaminidase; EC 220.127.116.11)
2-methyl-1,4-naphthoquinone sodium hydrogen sulfite (‘menadione sodium bisulfite’)
National Center for Biotechnology Information
open reading frame
peptide mass fingerprint
reactive oxygen species
real-time polymerase chain reaction
sodium dodecyl sulfate
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