Folia Microbiologica

, Volume 49, Issue 5, pp 519–525 | Cite as

Rice Na+/H+-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters

  • O. Kinclová-Zimmermannová
  • H. Flegelová
  • H. Sychrová
Article

Abstract

A triple mutant strain ofSaccharomyces cerevisiae lacking its own Na+-ATPases and Na+/H+ antiporters (ena1–4Δ nha1Δ nhx1Δ) was used for the expression of theOryza sativa NHX1 gene encoding a putative vacuolar Na+/H+ exchanger. Upon expression in yeast cells, theOsNhx1p is not a transport system specific only for sodium cations but it has a broad substrate specificity for at least four alkali metal cations (Na+, Li+, K+ and Rb+) and is able to substitute for the endogenous yeastScNhx1 antiporter. Its activity contributes to sequestration of alkali metal cations in intracellular vesicles.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2004

Authors and Affiliations

  • O. Kinclová-Zimmermannová
    • 1
  • H. Flegelová
    • 1
  • H. Sychrová
    • 1
  1. 1.Institute of PhysiologyAcademy of Sciences of the Czech RepublicPrague 4Czechia

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