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Folia Microbiologica

, Volume 50, Issue 5, pp 437–442 | Cite as

Use of green fluorescent protein as molecular marker for taggingBacillus brevis in soil under the control of a novel constitutive promoter F1

  • Yunpeng Chen
  • Daleng Shen
  • Mingjie Yang
Article

Abstract

A constitutive expression vector pHY300-F1gfp was constructed to test the function of promoter F1 subcloned from a rice epiphyteBacillus brevis strain DX01. The DX01 cells harboring plasmid pHY300-F1gfp were detected to produce bright green fluorescence. Subsequently, thegfp-taggedB. brevis strain was released into the soil and its survival was investigated by PCR and the detection of green fluorescence. The spatial location ofin situ gfp-tagged bacterial cells on the root surface of rice seedlings was visualized. All these results indicated that green fluorescent protein is an ideal molecular marker for the detection of the activities of promoter F1, and it is also a reliable probe to monitor specificB. brevis bacteria in the environment.

Keywords

Green Fluorescent Protein Bright Green Fluorescence Bleaching Powder Antibiotic Selective Pressure DX01 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  • Yunpeng Chen
    • 1
    • 2
  • Daleng Shen
    • 2
  • Mingjie Yang
    • 3
  1. 1.Department of Plant Science, School of Agriculture and BiologyShanghai Jiaotong UniversityShanghaiP.R. China
  2. 2.Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life SciencesFudan UniversityShanghaiP.R. China
  3. 3.Institute of Modern PhysicsFudan UniversityShanghaiP.R. China

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