Folia Microbiologica

, Volume 48, Issue 1, pp 111–115 | Cite as

Human tumor cells are selectively inhibited by colicins

Article

Abstract

The activityin vitro of four types of colicins (A, E1, E3, U) against one human standard fibroblast line and against 11 human tumor-cell lines carrying defined mutations of thep53 gene was quantified by MTT (tetrazolium bromide) assay. Flow cytometry showed that the pore-forming colicins A, E1 and U affected the cell cycle of 5 of these cell lines. Colicins E3 and U did not show any distinct inhibitory effects on the cell lines, while colicins E1 and especially A inhibited the growth of all of them (with one exception concerning colicin E1). Colicin E1 inhibited the growth of the tumor lines by 17–40% and standard fibroblasts MRC5 by 11%. Colicin A exhibited a differentiated 16–56% inhibition, the growth of standard fibroblasts being inhibited by 36%. In three of the lines, colicins A and E1 increased the number of cells in the G1 phase (by 12–58%) and in apoptosis (by 7–58%). These results correlated with the data from sensitivity assays. Hence, the inhibitory effect of colicins on eukaryotic cells is cell-selective, colicin-specific and can be considered to be cytotoxic.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2003

Authors and Affiliations

  1. 1.Department of Biology, Faculty of MedicineMasaryk UniversityBrnoCzechia

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