Enhancement of growth and secondary metabolite biosynthesis: Effect of elicitors derived from plants and insects

  • Gwi-Taek Jeong
  • Don-Hee Park
Article

Abstract

Plant-derived natural products have been and will continue to be valuable sources. Elicitors have been employed to modify cell metabolism in order to enhance the productivity of useful metabolites in plant cell/tissue cultures. In this study, several elicitors were used to improve the productivity of useful metabolites and to reduce culture time for archiving high concentration inP. ginseng hairy root cultures. The addition of chitosan, chitosan oligosaccharide and alginate oligosaccharide to the culture ofP. ginseng hairy roots caused growth to be inhibited with the increase in elicitor concentration. The usage of the chitosan elicitor andd-glucosamine caused a slight decrease in hairy root growth, whereas total ginseng saponin accumulated slightly with the increase in elicitor concentration. When gel beads were added to the culture medium at the initial period, hairy root growth was enhanced. The maximum growth was 1.35 times higher than that of the control at 1% (w/v). Total ginseng saponin content decreased due to the addition of alginate beads. This would result in consistent diffusion of lower levels of calcium ions during the culture period that promotes biomass growth.

Keywords

Panax ginseng hairy root elicitor chitosan alginate bead secondary metabolite 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2005

Authors and Affiliations

  • Gwi-Taek Jeong
    • 1
  • Don-Hee Park
    • 2
    • 3
    • 4
  1. 1.Engineering Research InstituteChonnam National UniversityGwangjuKorea
  2. 2.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuKorea
  3. 3.Faculty of Applied Chemical EngineeringChonnam National UniversityGwangjuKorea
  4. 4.Biotechnology Research InstituteChonnam National UniversityGwangjuKorea

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