Folia Microbiologica

, Volume 31, Issue 1, pp 19–31

Mode of action of the antimicrobial compound 5-Bromo-5-nitro-1,3-dioxane (Bronidox)

  • M. Ghannoum
  • M. Thomson
  • W. Bowman
  • S. Al-Khalil
Article

DOI: 10.1007/BF02928676

Cite this article as:
Ghannoum, M., Thomson, M., Bowman, W. et al. Folia Microbiol (1986) 31: 19. doi:10.1007/BF02928676

Abstract

The mode of action of the antimicrobial agent, 5-bromo-5-nitro-l,3-dioxane (bronidox), was studied in detail for gram-positive and gram-negative bacteria, yeast and fungi. The studies included MIC testing, thiol inhibition of activity, intracellular leakage, oxygen consumption, incorporation of3H-uridine, scanning electron microscopy, inhibition of enzyme activity (papain) andin vitro oxidation of thiols to disulfides. It appears that the primary mode of action of bronidox is the same as, or similar to, that of bronopol,i.e. the oxidation of essential protein thiol causing inhibition of enzyme activity and subsequent inhibition of microbial growth.

Abbreviations

PCMB

4-chloromercuribenzoic acid

PMSF

phenylmethanesulfonyl fluoride

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1986

Authors and Affiliations

  • M. Ghannoum
    • 1
  • M. Thomson
    • 2
  • W. Bowman
    • 3
  • S. Al-Khalil
    • 3
  1. 1.Department of Botany and MicrobiologyKuwait University, Faculty of ScienceKuwait
  2. 2.Department of BiochemistryKuwait University, Faculty of ScienceKuwait
  3. 3.Department of ChemistryUniversity of TechnologyLoughboroughUK

Personalised recommendations