Aeration sensitizesStreptococcus faecalis to hydroxyurea
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Abstract
Hydroxyurea in up to 60mm concentration did not inhibit growth or DNA synthesis in nonaerated cultures ofStreptococcus faecalis ATCC 8043. In contrast, in cultures aerated by shaking already 1mm hydroxyurea decreased the rate of net DNA synthesis and in higher concentrations of the drug the growth of the total cell mass also slowed down and the number of cells per chain increased from 1 – 2 to 10. The differential rate of DNA synthesis, but not the growth of the total cell mass, could be restored almost to the control level by adding thymidine to the medium. Thus there are at least two targets for hydroxyurea in the cells ofS. faecalis grown in aerated cultures.
Keywords
Thymidine Hydroxyurea Differential Rate Ribonucleotide Reductase Dolin
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References
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© Institute of Microbiology, Academy of Sciences of the Czech Republic 1979