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Factors affecting protein synthesis during biotin deficiency inAspergillus nidulans

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Abstract

Biotin deficiency inAspergillus nidulans resulted in a 70 % increase of the protein content and increased levels of free and bound aspartate, glutamate, serine, leucine and methionine. Likewise, the activities of NADP+ glutamate dehydrogenase, NAD+ glutamate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were significantly increased. The total UNA content increased while the DNA content was unaffected. The rRNA/tRNA ratio remained higher in biotin-deficient cells. Supplementation of glutamate, aspartate, serine, leucine and methionine to the culture medium raised the rRNA/tRNA ratio, and the difference observed in the qualitative and the quantitative patterns of protein and dry cell mass between normal and biotin-deficient cultures was abolished.

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Author notes

  1. J. D. Desai

    Present address: Department of Biosciences, Sardar Patel University, 388120, Vallabh vidyanagar, India

Authors and Affiliations

  1. Department of Microbiology,Faculty of Science, MS University of Baroda, 390002, India

    J. D. Desai

  2. Department of Microbiology, MS University of Baroda, India

    V. V. Modi

Authors
  1. J. D. Desai
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  2. V. V. Modi
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Additional information

The award of a Research Fellowship from The Government of India, Department of Atomic Energy, Bombay is gratefully acknowledged. I wish to thank Prof. V. V. Modi, Head, Department of Microbiology, M.S. University of Baroda, for his valuable guidance during this investigation.

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Desai, J.D., Modi, V.V. Factors affecting protein synthesis during biotin deficiency inAspergillus nidulans . Folia Microbiol 24, 379–384 (1979). https://doi.org/10.1007/BF02927119

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