International Journal of Pancreatology

, Volume 11, Issue 2, pp 109–116

HPLC and FPLC

Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins
  • Burkhard Göke
  • Volker Keim
Article

Summary

Automated chromatography techniques such as high-performance (high-pressure) liquid chromatography (HPLC) or fast protein liquid chromatography (FPLC) represent a promising, new approach toward resolving the complex protein mixture of pancreatic juice. To achieve highly reproducible and rapid resolution of pancreatic protein profiles, reversed-phase, hydrophobic interaction, and ion-exchange techniques are employed. Until now, none of the respectively reported methods has provided a convenient, specific procedure for diagostic purposes. However, experimental data prove that these methods are very sensitive and furthermore, easy to handle. Therefore, it seems possible to utilize such methods for diagnostics in future.

Key Words

Pancreatic juice enzymes HPLC FPLC 

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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Burkhard Göke
    • 1
  • Volker Keim
    • 2
  1. 1.Zentrum Innere MedizinAbt. Gastroenterologie der Philipps Universität MarburgMarburgGermany
  2. 2.Medizinische Klinik IV, GastroenterologieKlinikum Mannheim, Universität HeidelbergMannheimGermany

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