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Folia Microbiologica

, Volume 33, Issue 6, pp 462–465 | Cite as

Multiple populations of double-stranded RNA in two virus-harbouring strains ofTrichomonas vaginalis

  • J. Flegr
  • J. Čerkasov
  • J. Štokrová
Article

Abstract

The existence of six dsRNA segments ofTrichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsRNA serving as a standard. TheM’s were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 Mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. Cell homogenate fractionation showed that all dsRNA segments were associated with viral particles. The data appeared to support the hypothesis of a relationship between viruses of the protozoanT. vaginalis and of the yeastSaccharomyces cerevisiae.

Keywords

Viral Particle Molar Mass Ammonium Sulfate Guanidine Hydrochloride Trichomoniasis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1988

Authors and Affiliations

  • J. Flegr
    • 1
  • J. Čerkasov
    • 2
  • J. Štokrová
    • 1
  1. 1.Institute of Molecular GeneticsCzechoslovak Academy of SciencesPrague 6
  2. 2.Faculty of ScienceCharles UniversityPrague 2

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