Skip to main content
SpringerLink
Log in

Antiproliferative effects of natural human tumor necrosis factor-α, interferon-α, and interferon-γ on human pancreatic carcinoma cell lines

  • Published:
International journal of pancreatology Aims and scope Submit manuscript

Summary

The antiproliferative effects of natural human tumor necrosis factor-α (nHuTNF-α), natural human interferon-α (nHuIFN-α), and natural human interferon-γ (nHuIFN-γ) were investigated in human pancreatic carcinoma cell lines. HuP-T3, HuP-T4, and BxPC-3 carcinoma cells exhibited mild growth inhibition after treatment with nHuTNF-α. HuP-T3, HuP-T4, MIA-PaCa-2, and BxPC-3 cells exhibited mild or marked growth inhibition after treatment with nHuIFN-α. Incubation with nHuIFN-γ caused marked growth inhibition of HuP-T4 and BxPC-3 cells, whereas HuP-Tl, HuP-T3, and MIA-PaCa-2 cells showed only mild growth inhibition with the same dose of nHuIFN-7. Combined nHuTNF-a and nHuIFN-a (1:1) demonstrated marked synergism in comparison with their effects as single agents on HuP-Tl, HuP-T3, and MIA-PaCa-2 cells. The combination of nHuTNF-a and nHuIFN-7 (100:1) also demonstrated a marked synergistic effect in comparison with these cytokines alone in four out of five pancreatic carcinoma cell lines (HuP-T1, HuP-T3, HuP-T4, and MIA-PaCa-2 cell lines). The marked increase in efficacy brought about by using combinations of cytokines may enable some improvement in the treatment of pancreatic carcinoma patients in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dubois MF, Ferrieux C, Lebon P. Synergistic cytotoxic effects of recombinant human tumor necrosis factor, interferon, and heat-stress. Cancer Res. 1989; 49: 5618–5622.

    PubMed  CAS  Google Scholar 

  2. Orita K, Ando S, Kurimoto M. Synergism between human tumor necrosis factor and human interferon-α. Acta. Med. Okayama 1987; 41: 155–160.

    PubMed  CAS  Google Scholar 

  3. Shiiki S, Fuchimoto S, Orita K. Therapeutic potential of natural human tumor necrosis factor-α in combination with natural human interferon-α or -γ on human cancer cells. Res. Commun. Chem. Pathol. Pharm. 1988; 61: 345–351.

    CAS  Google Scholar 

  4. Shiiki S, Fuchimoto S, Orita K. Synergistic antiproliferative effects of natural human tumor necrosis factor-α and natural human interferon-α or -γ on human cancer cell lines. Res. Commun. Chem. Pathol. Pharm. 1989; 64: 87–97.

    CAS  Google Scholar 

  5. Helseth E. Torp S, Dalen A, Unsgaad G. Effects of interferon-gamma and tumor necrosis factor-alpha on clonogenic growth of cell lines and primary cultures from human gliomas and brain metastasis. APMIS 1989; 97: 569–574.

    Article  PubMed  CAS  Google Scholar 

  6. Nishiyama Y, Fuchimoto S, Orita K. Preventive and antiproliferative effects of tumor necrosis factor against experimental hepatic metastases of mouse colon-26 tumor. Jpn. J. Cancer Res. 1989; 80: 366–372.

    Google Scholar 

  7. Kuhl JS, Klapdor R, Bahlo M, Franke N, Kunde C, Arps H, Dietel M. Cytokines and pancreatic cancer: Sensitivity of xenotransplants of pancreatic carcinomas to rIFN-γ and rTNF-α in nude mice. Int. J. Pancreatology 1989; 4: 303–319.

    CAS  Google Scholar 

  8. Schmeigel WH, Caesar J, Kalthoff H, Greten H, Schreber HW, Thiele H-G. Antiproliferative effects exerted by recombinant human tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) on human pancreatic tumor cell lines. Pancreas 1988; 3: 180–188.

    Article  Google Scholar 

  9. Fukuda S, Yamane Y, Kouguchi M, Ando S, Sato Y, Kurimoto M. Purification and characterization of natural human tumor necrosis factor from HVJ-stimulated BALL-1 cells. Chem. Express 1987; 2: 101–104.

    CAS  Google Scholar 

  10. Ando S, Sano O, Fujii M, Sato Y, Kurimoto M. Large scale production and characterization of natural human interferon-α from HVJ-stimulated BALL-1 cells. Chem. Express 1987; 2: 45–48.

    CAS  Google Scholar 

  11. Ando S, Ohta T, Tanimoto T, Sano O, Yamauchi H, Ando O, Torigoe K, Kurimoto M. Natural human interferon-γ derived from lipopolysaccharide-stimulated human myelomonocytic HBL-38 cells. Jpn. J. Cancer Res. (Gann) 1988; 79: 757–765.

    CAS  Google Scholar 

  12. Lee S, Aggarwal BB, Rinderknecht C, Assisi F, Chiu H. The synergistic anti-proliferative effect of γ-interferon and human lymphotoxin. J. Immunol. 1984; 133: 1083–1086.

    PubMed  CAS  Google Scholar 

  13. Chou TC, Talalay P. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs of enzyme inhibitors. Adv. Enzyme Reguln. 1984, 27–48.

  14. Tsujimoto M, Yip YK, Vilćek J. Interferon-γ enhances expression of cellular receptors for tumor necrosis factor. J. Immunol. 1986; 136: 2441–2444.

    PubMed  CAS  Google Scholar 

  15. Aggarwal BB, Eessalu TE, Hass PE. Characterization of receptors for human tumor necrosis factor and their regulation by γ-interferon. Nature 1985; 318: 665–667.

    Article  PubMed  CAS  Google Scholar 

  16. Ruggaiero V, Tarernier J, Fiers W, Baglioni C. Induction of the synthesis of tumor necrosis factor receptors by interferon-γ. J. Immunol. 1986; 136: 2445–2450.

    Google Scholar 

  17. Tsujimoto M, Vilćek J. Tumor necrosis factor receptor in HeLa cells and their regulation by interferon-γ. J. Biol. Chem. 1986; 261: 5384–5388.

    PubMed  CAS  Google Scholar 

  18. Sumi C, Brinck-Johnsen T, Longnecker DS. Inhibition of a transplantable pancreatic carcinoma by castration and estradiol administration in rats. Cancer Res. 1989; 49: 6687–6692.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, 700, Okayama, Japan

    Nagahide Matsubara, Sadanori Fuchimoto & Kunzo Orita

Authors
  1. Nagahide Matsubara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sadanori Fuchimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kunzo Orita
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsubara, N., Fuchimoto, S. & Orita, K. Antiproliferative effects of natural human tumor necrosis factor-α, interferon-α, and interferon-γ on human pancreatic carcinoma cell lines. Int J Pancreatol 8, 235–243 (1991). https://doi.org/10.1007/BF02924542

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02924542

Key Words

Search

Navigation