Folia Microbiologica

, Volume 30, Issue 3, pp 212–223 | Cite as

Antigenic properties of T cell antigen-specific receptors isolated from the surface of rabbit and mouse spleen and lymph node cells

  • J. Rejnek
  • L. Tučková
  • J. Zikán
  • B. Říhová
  • J. Kostka
Article
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Abstract

The presence ofa allotypic determinants was tested in fractions obtained by gel filtration of antigen-specific receptors isolated by immunoadsorption from lymphoid cells of antigen-stimulateda3-3 rabbits. This technique, as well as the inhibition of the reaction of isolated receptors with anti-T cell receptor antisera (anti R) by anti-a3 antibodies failed to demonstrate the presence of a allotypie determinants. The inhibitory effect of antigen-specific receptors isolated from the lymphoid cells of stimulated A/J mice on the cytotoxic effect of anti-Ia antibodies on mouse spleen cells in the presence of rabbit complement was tested. All preparations inhibited the cytotoxic reaction with the average effectivity of 60%. In order to confirm the presence of Ia determinants on the rabbit and mouse T cell receptor molecules it was shown that the reactions of three anti-R antisera with 12 different receptor preparations were inhibited by anti-la antibodies. SDS-PAGE analyses of125I-labelled mouse specific receptors and the precipitate obtained by anti-R antisera showed that T cell receptors were present in fractions with molar mass 100 and 85 kg/mol. The molar mass of the former fraction after reduction and alkylation was 45 kg/mol.

Keywords

Major Histocompatibility Complex Rabbit Complement Receptor Preparation Idiotypic Determinant Arsanilic Acid 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1985

Authors and Affiliations

  • J. Rejnek
    • 1
  • L. Tučková
    • 1
  • J. Zikán
    • 1
  • B. Říhová
    • 1
  • J. Kostka
    • 1
  1. 1.Department of Immunology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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