Response of “storage” effects in barley seeds to the dose of ethyl methanesulphonate and X-ray treatment
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1. Ethyl methanesulphonate (BMS)-treated barley seeds stored at 30 % water content for 1 – 4 weeks are capable of recovering from the mutagen induced injury. The level and speed of the recovery depends on the EMS dose and storage period. Seeds treated with extremely severe EMS doses reducing the% of germination to 5 – 10 % cannot recover from the induced injury. 2. Due to a 1 to 6 weeks storage of EMS-treated seeds with 20 % water content the amount of induced injury increases in dependence on the applied EMS dose and storage period. Doses of EMS, reducing the seedling height in the M1 generation to 90 – 95 % of the control reveal no storage after-effects. 3. Storage of X-ray exposed seeds at 20 % or 30 % water content did not result in any significant changes in the yield of induced genetic damage.
KeywordsStorage Period Seed Storage Barley Seed Seedling Height Methanesulphonate
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- Ehrenberg, L.: Radiobiological mechanisms of genetic effects. A review of some current lines of research. - Radiat. Res. (Suppl.)1: 102–123, 1959.Google Scholar
- Howland, G. P., HaRt, R. W.: Radiation biology of cultured plant cells. - In:Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspacts of Plant Cell, Tissue, and Organ Culture. Pp. 731–754. Springer Verlag, Berlin, Heidelberg, New York 1977.Google Scholar
- Strauss, B., Coyle, M., Mc Mahon, M., Kato, K., Dolyniuk, M.: DNA synthesis repair and chromosome breaks in eucaryotic cell. - In:Beers, R. F.,Herriott, R. M.,Tilghman, R. C. (ed.): Molecular and Cellular Repair Processes. - Johns Hopkins Med. J. (Suppl.) 1: 111 to 124, 1971.Google Scholar
- Velemínský, J., Gichner, T.: Recovery from mutagen-induced injury and DNA repair in barley. - In:Gaul, H. (ed.): Barley Geneties. III. Proc. 3-rd International Barley Genetics Symposium. Pp. 146–154. Garching 1976.Google Scholar