The effects of nutrients and temperature on biomass, growth, lipid production, and fatty acid composition ofCyclotella cryptica Reimann, Lewin, and Guillard
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Batch cultures ofCyclotella cryptica Reimann, Lewin, and Guillard were grown at two different nutrient (nitrogen/silica) levels and at two different temperatures (20 and 30°C). Biomass and cell yields decreased with decreased levels of both the nutrients (nitrogen and silica) at 20 and 30°C; whereas lipids (total, neutral, and polar) increased with decreased levels of nutrients at these two temperatures. Changes in fatty acid composition were also noted; the diatoms produced increased amounts of fatty acids C14:0 and C16:0 when grown in nutrient-deficient medium. The influence of nutrient stress and temperature is discussed.