Biologia Plantarum

, Volume 18, Issue 5, pp 401–407 | Cite as

Effect of cannabis (hashish) on mitosis ofallium cepa L. root tips

  • A. Kabaeity
  • A. El-Bayoumi
  • A. Habib


Treatment with cannabis induces a partial inactivation of the spindle apparatus, and an unequal separation of the chromosomes. There is more or less a tendency to с-mitosis at higher concentrations and to multipolar anaphases at lower concentrations like so many other narcotic substances. The MI value decreases linearly and sharply with the increase of concentrations used,i.e. cannabis had a severe mitodepressive effect. Long treatment for 24 h caused the death of all colls examined.

Cannabis causes a complete dissolution of the matrix substance of the chromosomes and breaks down the chromatin material of the nucleus to minute granules. This phenomenon was observed in all root tips treated with the cannabis. The same picture was observed after letting the roots recover in water.

The application of cannabis may disturb the extrachromosomal mechanism. This disturbance is restricted to the abnormal diffusion of the spindle organizers in many directions instead of two poles.


Mitotic Index Multinucleate Cell Applied Concentration Spindle Fibre Abnormal Diffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Darlington, C. D.: The external mechanics of the chromosomes. I. The scope of enquiry. — Proc. roy. Soc. London, Ser. B121 : 264–319, 1936.CrossRefGoogle Scholar
  2. Davidson, D., Macleod, R. D.: Changes in mitotic indices in roots ofVicia faba. I. Antagonistic effects of colchicine and IAA. — Chromosoma18 : 421–437, 1966.CrossRefPubMedGoogle Scholar
  3. Deysson, G.: Antimitotic substances. — Int. Rev. Cytol.24 : 99–148, 1968.CrossRefPubMedGoogle Scholar
  4. Kabarity, A.: Induction of multipolare spindles in the meiosis ofTriticum aestivum as affected by acetone. — Cytologia31 : 457–460, 1966.CrossRefGoogle Scholar
  5. Levan, A.: Effect of colchicine on root mitosis inAllium. — Hereditas24 : 471–486, 1938.CrossRefGoogle Scholar
  6. Levan, A.: Colchieine-induced с-mitosis in two mouse ascite.s tumours. — Hereditas40 : 1–64, 1954.CrossRefGoogle Scholar
  7. Östergken, G.: C-mitosis, chromosome concentration, narcosis and protein chain folding. — Hereditas30 : 429–467, 1944.CrossRefGoogle Scholar
  8. Östergren, G.: Cytological standards for the quantitative estimation of spindle disturbances. — Hereditas36 : 371–382, 1950.CrossRefGoogle Scholar
  9. Therman, E., Timonen, S.: Multipolar spindles in human cancer cells. — Hereditas36 : 393 to 405, 1950.Google Scholar
  10. Webster, P. L., Davidson, D.: Changes in the duration of the mitotic cycle induced by colchicine and indole-3-acetic acid inVicia faba roots. — J. exp. Bot.20 : 671–685, 1969.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 1976

Authors and Affiliations

  • A. Kabaeity
    • 1
  • A. El-Bayoumi
    • 1
  • A. Habib
    • 1
  1. 1.Botany Department Faculty of ScienceKuwait University — State of Kuwait and Ain Shams UniversityCairoEgypt

Personalised recommendations