Applied Biochemistry and Biotechnology

, Volume 32, Issue 1–3, pp 149–158 | Cite as

Activation and detection of (Pro)mutagenic chemicals using recombinant strains ofStreptomyces griseus

  • Steven E. Buchholz
  • Charles A. Omer
  • Paul V. Viitanen
  • F. Slma Sariaslani
  • Ralph G. Stahl


Two recombinant strains ofStreptomyces griseus have been developed to report on the activation of promutagenic° chemicals. This activation is monitored by reversion of the bacterial test strains to a kana-mycin-resistant phenotype. Strain H69 detects point mutations and was reverted at an increased frequency by acetonitrile, 2-aminoanthracene, 1,2-benzanthracene, benzidine, benzo(a)pyrene, 9,10-dimethyl-1,2-benzanthracene, and glycine. The second strain, FS2, detects frame shift mutations and was reverted at an increased frequency by 1,2-benzanthracene, benzidine, and glycine. Compounds such as butylated hydroxytoluene, catechol, chlorobenzene, hydroquinone, potassium chloride, phenol,cis-stilbene,trans-stilbene, and toluene did not elicit positive responses in either strain. In addition, these strains are capable of detecting direct-acting mutagens such asN-methyl-N’-nitrosoguanidine and ICR-191, providing further evidence of their promise for detecting a wider range of mutagens. To our knowledge, this is the first report of bacterial strains capable of activating promutagenic compounds and detecting their mutagenic metabolites without the benefit of an exogenous activation system such as the rodent liver homogenate (S9).

Index Entries

Streptomyces griseus cytochrome P-450 activation of promutagens detection of mutagens 


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  1. 1.
    Ames, B. N., McCann, J., and Yamasaki, E. (1975),Mutation Res. 31, 347.Google Scholar
  2. 2.
    Ashby, J. and Tennant, R. W. (1988),Mutation Res. 204, 17.CrossRefGoogle Scholar
  3. 3.
    Auletta, A. and Ashby, J. Workshop on the relationship between short-term test information and carcinogenicity, Williamsburg, VA (January, 1988), pp. 20–23.Google Scholar
  4. 4.
    BBN (1984),RS/1 User’s Guide, Book 2: Graphics and Statistics, BBN Software Products Corp., Cambridge, MA, pp.Google Scholar
  5. 5.
    Beck, E., Lugwig, G., Auerswald, E. A., Reiss, B., and Schaller, H. (1982),Gene. 19, 327.CrossRefGoogle Scholar
  6. 6.
    Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heynecker, H. L., Boyer, H. W., Crosa, J. H., and Falkow, S. (1977),Gene. 2, 95.CrossRefGoogle Scholar
  7. 7.
    Carter, P., Bedouelle, H., Waye, M., and Winter, G. (1985),Oligonucleotide Site-Directed Mutagenesis in M13, Anglian Biotechnology, London, pp.Google Scholar
  8. 8.
    Casadaban, M. J., Martinez-Arias, A., Shapira, S. K., and Chou, J. (1983),Meth. Enzymol. 100, 293.CrossRefGoogle Scholar
  9. 9.
    Cerniglia, C. E., White, G. L., and Heflich, R. H. (1985),Arch. Microbiol. 143, 105.CrossRefGoogle Scholar
  10. 10.
    Claxton, L. D., Allen, J., Auletta, A., Morelmanns, K., Nestmann, E., and Zeiger, E. (1987),Mutation Res. 189, 83.CrossRefGoogle Scholar
  11. 11.
    Gold, L. S., Slone, T. H., and Berstein, L. (1989),Environ. Health Perspect. 79, 259.CrossRefGoogle Scholar
  12. 12.
    Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. (1983),Environ. Muta. 5(Suppl. 1), 3.CrossRefGoogle Scholar
  13. 13.
    Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Burton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., and Ward, J. M. (1985),Genetic Manipulation of Streptomyces: A Laboratory Manual, John Innes Foundation, Norwich, UK, pp.Google Scholar
  14. 14.
    Katz, E., Thompson, C. J., and Hopwood, D. A. (1983),J. Gen. Microbiol. 129, 2703.Google Scholar
  15. 15.
    McCoy, E. C., Speck, W. T., and Rosenkranz, H. S. (1977),Mutation Res. 46, 261.Google Scholar
  16. 16.
    Maron, D. M. and Ames, B. N. (1983),Mutation Res. 113, 173.Google Scholar
  17. 17.
    Nestmann, E. R., Brillinger, R. L., McPherson, M. F., and Maus, K. L. (1987),Environ. Mol. Mutagen. 10, 169.CrossRefGoogle Scholar
  18. 18.
    Pugsley, A. P. and Oudega, B. (1987),Plasmids: A Practical Approach, Hardy, K.G., ed., IRL, Washington, DC, pp. 105–161.Google Scholar
  19. 19.
    Quillardet, P., Bellecombe, C., and Hofnung, M. (1985),Mutation Res. 147, 79.Google Scholar
  20. 20.
    Rapson, W. H., Nazar, M. A., and Butsky, V. V. (1980),Bull. Environ. Contarn. Toxicol. 24, 590.CrossRefGoogle Scholar
  21. 21.
    Sarkar, H. K., Viitanen, P. V., Padan, E., Trumble, W. R., Poonian, M. S., McComas, W., and Kaback, H. R. (1986),Meth. Enzy. 125, 214.CrossRefGoogle Scholar
  22. 22.
    Sariaslani, F. S. and Kunz, D. A. (1986),Biochem. Biophys. Res. Comm. 141, 405.CrossRefGoogle Scholar
  23. 23.
    Sariaslani, F. S. and Stahl Jr., R. G. (1990),Biochem. Biophys. Res. Comm. 166, 743.CrossRefGoogle Scholar
  24. 24.
    Steel, R. G. D. and Torrie, J. H. (1960),Principles and Procedures of Statistics, McGraw Hill, New York, pp.Google Scholar
  25. 25.
    Trower, M. K., Sariaslani, F. S., and Kitson, F. G. (1988),Biochem. Biophys. Res. Comm. 157, 1417.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Steven E. Buchholz
    • 1
  • Charles A. Omer
    • 1
  • Paul V. Viitanen
    • 1
  • F. Slma Sariaslani
    • 1
  • Ralph G. Stahl
    • 2
  1. 1.Central Research and DevelopmentExperimental Station and E. I. du Pont de Nemours & CoWilmington
  2. 2.Central Research and DevelopmentHaskell Laboratory for Toxicology and Industrial MedicineWilmington

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