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Applied Biochemistry and Biotechnology

, Volume 32, Issue 1–3, pp 149–158 | Cite as

Activation and detection of (Pro)mutagenic chemicals using recombinant strains ofStreptomyces griseus

  • Steven E. Buchholz
  • Charles A. Omer
  • Paul V. Viitanen
  • F. Slma Sariaslani
  • Ralph G. Stahl
Article

Abstract

Two recombinant strains ofStreptomyces griseus have been developed to report on the activation of promutagenic° chemicals. This activation is monitored by reversion of the bacterial test strains to a kana-mycin-resistant phenotype. Strain H69 detects point mutations and was reverted at an increased frequency by acetonitrile, 2-aminoanthracene, 1,2-benzanthracene, benzidine, benzo(a)pyrene, 9,10-dimethyl-1,2-benzanthracene, and glycine. The second strain, FS2, detects frame shift mutations and was reverted at an increased frequency by 1,2-benzanthracene, benzidine, and glycine. Compounds such as butylated hydroxytoluene, catechol, chlorobenzene, hydroquinone, potassium chloride, phenol,cis-stilbene,trans-stilbene, and toluene did not elicit positive responses in either strain. In addition, these strains are capable of detecting direct-acting mutagens such asN-methyl-N’-nitrosoguanidine and ICR-191, providing further evidence of their promise for detecting a wider range of mutagens. To our knowledge, this is the first report of bacterial strains capable of activating promutagenic compounds and detecting their mutagenic metabolites without the benefit of an exogenous activation system such as the rodent liver homogenate (S9).

Index Entries

Streptomyces griseus cytochrome P-450 activation of promutagens detection of mutagens 

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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Steven E. Buchholz
    • 1
  • Charles A. Omer
    • 1
  • Paul V. Viitanen
    • 1
  • F. Slma Sariaslani
    • 1
  • Ralph G. Stahl
    • 2
  1. 1.Central Research and DevelopmentExperimental Station and E. I. du Pont de Nemours & CoWilmington
  2. 2.Central Research and DevelopmentHaskell Laboratory for Toxicology and Industrial MedicineWilmington

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