Applied Biochemistry and Biotechnology

, Volume 31, Issue 3, pp 273–282 | Cite as

Organosolv-pulping III

The influence of Formic acid delignification on the enzymatic hydrolysis ofPinus radiata D. Don Sawdust
  • Jaime Baeza
  • Ana Maria Fernández
  • Juanita Freer
  • Ana Pedreros
  • Eckhard Schmidt
  • Nelson Durán
Article
Received:
Accepted:

Abstract

Formic acid pretreatment onPinus radiata D. Don was studied in order to improve the cellulose hydrolysis by commercial cellulase. The formic acid treatment effectively removed the lignin. A low substitution (formylation) and a crystallinity decrease of the cellulose in the pulp were observed. As consequence of these parameter changes, owing to the formic acid pretreatment on sawdust, a higher saccharification value was observed. The degree of saccharification increased when the degree of substitution (measured by titration) decreased and the portion of amorphous cellulose (measured via an X-ray technique) increased.Trichoderma reesei cellulase hydrolyzed the untreated and pretreated Pinus sawdust with formic acid in 25% and 56% of saccharification, respectively.

Index Entries

Organosolv pulping enzymatic hydrolysis formic acid delignification Pinus radiata 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Johansson, A., Aaltonen, O., and Ylinen, P. (1987),Biomass 13, 45–65.CrossRefGoogle Scholar
  2. 2.
    Aziz, S., McDonough, T., Thompson, N., and Doshi, M. R. (1988),TAPPI J. 71, 251–254.Google Scholar
  3. 3.
    Baeza, J., Freer, J., Pedreros, A., Schmidt, E., Mansilla, H., and Durán, N. (1990),Bol. Soc. Chil. Quitn. 35, 331–337.Google Scholar
  4. 4.
    Freudenberg, K., Janson, A., Knopf, E. and Haag, A. (1936),Berichte 69B, 1415–1425.Google Scholar
  5. 5.
    Staudinger, H. and Dreher, E. (1936),Berichte 69B, 1729–1739.Google Scholar
  6. 6.
    Wright, G. F. and Hibbert, H. (1937),J. Am. Chem. Soc. 59, 125–130.CrossRefGoogle Scholar
  7. 7.
    Bucholtz, M. and Jordan, R. K. (1983),Pulp and Paper 57, 102–104.Google Scholar
  8. 8.
    Jordan, R. K. (1982),PCT Int. Appl. WO 8201, 902.Google Scholar
  9. 9.
    Baeza, J., Freer, J., Pedreros, A., and Belmar, H. (1987), Proc. VI National Meeting of Research and Development of Forestry Products, Chile, pp. 163–174.Google Scholar
  10. 10.
    Sapag-Hagar, J., Dimitroff, N., and Piffaut, O. (1985), Proc. Symposium of Chemistry and National Development Chilean Chem. Soc., July 1985, Concepción, Chile, pp. 132ó136.Google Scholar
  11. 11.
    Mamers, H. and Menz, D. N. J. (1984),APPITA 37, 644–649.Google Scholar
  12. 12.
    Clark, T. A. and Mackie, K. L. (1987),J. Wood Chem. Technol. 7, 373–403.CrossRefGoogle Scholar
  13. 13.
    Hohlberg, A. I., Aguilera, J. M., Agosin, E., and San Martin, R. (1989),Biomass 18, 81–93.CrossRefGoogle Scholar
  14. 14.
    Dekker, R. F. H. (1987),Biocatalysis 1, 63–75.CrossRefGoogle Scholar
  15. 15.
    Baeza, J., Freer, J., Palma, G., Pedreros, A., Rodriguez, J., Rojas, N., and Durán, N. (1985), Proc. Symposium of Chemistry and Renewable Resources Utilization, Chilean Chem. Soc., July 1985, Concepcián, Chile, pp. 63–66.Google Scholar
  16. 16.
    Baeza, J., Freer, J., Pedreros, A., and Durán, N. (1988), Proc. XVIII Latin American Chemical Congress, January 1988, Santiago, Chile, pp. 513, 514.Google Scholar
  17. 17.
    Durán, N., Ferraz, A., Freer, J., Pedreros, A., and Baeza, J. (1988), FENABIO-88, First National Conference of Biotechnology, April 1988, R. J. Brazil.Google Scholar
  18. 18.
    Fujimoto, T., Takahashi, S.-I., Tsuji, M., Miyamoto, T., and Inagaki, H. (1986),J. Polym. Sci. Part C, Polym. Lett. 24, 495–501.CrossRefGoogle Scholar
  19. 19.
    Gharpuray, M. M., Lee, Y. H., and Fan, L. T. (1983),Biotechnol. Bioeng. 25, 157–172.CrossRefGoogle Scholar
  20. 20.
    Grethlein, H. E. (1985),Bio/Technology, Feb. pp. 155–160.Google Scholar
  21. 21.
    Mandels, M., Andreotti, R., and Roche, C. (1976),Biotechnol. Bioeng. Symp. 6, 21–33.Google Scholar
  22. 22.
    Commission on Biotechnology, IUPAC (1987),Pure & Appl. Chem. 59, 245–256.CrossRefGoogle Scholar
  23. 23.
    Miller, G. L. (1959),Anal. Chem. 31, 426–428.CrossRefGoogle Scholar
  24. 24.
    Updegraff, D. M. (1969),Anal. Biochem. 32, 420–424.CrossRefGoogle Scholar
  25. 25.
    TAPPI Standard T-222 OM-88. Acid-Insoluble lignin in Wood and Pulp 1988.Google Scholar
  26. 26.
    Simionescu, C. I., Popa, V. I., Rusan, V., and Rusan, M. (1985),Cellulose Chem. Technol. 19, 525–530.Google Scholar

Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Jaime Baeza
    • 1
  • Ana Maria Fernández
    • 1
  • Juanita Freer
    • 1
  • Ana Pedreros
    • 1
  • Eckhard Schmidt
    • 1
  • Nelson Durán
    • 2
  1. 1.Departmento de Quimica, Renewable Resources LaboratoryUniversidad de ConceptionConceptionChile
  2. 2.Instituto de Quimica, Biological Chemistry LaboratoryUniversidade Estadual de CampinasCampinasBrazil

Personalised recommendations