Applied Biochemistry and Biotechnology

, Volume 31, Issue 3, pp 273–282

Organosolv-pulping III

The influence of Formic acid delignification on the enzymatic hydrolysis ofPinus radiata D. Don Sawdust
  • Jaime Baeza
  • Ana Maria Fernández
  • Juanita Freer
  • Ana Pedreros
  • Eckhard Schmidt
  • Nelson Durán
Article

Abstract

Formic acid pretreatment onPinus radiata D. Don was studied in order to improve the cellulose hydrolysis by commercial cellulase. The formic acid treatment effectively removed the lignin. A low substitution (formylation) and a crystallinity decrease of the cellulose in the pulp were observed. As consequence of these parameter changes, owing to the formic acid pretreatment on sawdust, a higher saccharification value was observed. The degree of saccharification increased when the degree of substitution (measured by titration) decreased and the portion of amorphous cellulose (measured via an X-ray technique) increased.Trichoderma reesei cellulase hydrolyzed the untreated and pretreated Pinus sawdust with formic acid in 25% and 56% of saccharification, respectively.

Index Entries

Organosolv pulping enzymatic hydrolysis formic acid delignification Pinus radiata 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Humana Press Inc. 1991

Authors and Affiliations

  • Jaime Baeza
    • 1
  • Ana Maria Fernández
    • 1
  • Juanita Freer
    • 1
  • Ana Pedreros
    • 1
  • Eckhard Schmidt
    • 1
  • Nelson Durán
    • 2
  1. 1.Departmento de Quimica, Renewable Resources LaboratoryUniversidad de ConceptionConceptionChile
  2. 2.Instituto de Quimica, Biological Chemistry LaboratoryUniversidade Estadual de CampinasCampinasBrazil

Personalised recommendations