Biologia Plantarum

, Volume 36, Issue 4, pp 527–534 | Cite as

Micropropagation ofAsparagus cooperi as affected by growth regulators

  • B. Ghosh
  • S. Sen
Orginal Papers


For clonal propagation ofAsparagus cooperi, shoot tips and node explants of 7, 20 and 35 d old spear from the region within 10 cm and below 25 cm from the apex were cultured in modified Murashige and Skoog's (1962) medium containing 6-benzylaminopurine (BAP). The required concentration of BAP varied in explants of different ages and types. In shoot tip culture, the rate of shoot multiplication was higher after 40 d than 60 d of culture. The maximum mumber (62–65) of shoots were obtained from shoot tip explants of 20 d old spear in the medium containing 2.0 mg dm−3 of BAP, 80 mg dm−3 of adenine and 0.02 mg dm−3 of α-naphthalene acetic acid after 60 d of culture. From node cultures, high number of shoots were obtained after 30 d. Pretreatment with BAP in liquid medium for 48 h was effective for semirejuvenescence. The individual shoots produced roots in presence of indole-3-butyric acid and also in potassium salt of indole-3-butyric acid, the later being more effective. All regeneratns were cytologically stable.





indole-3-butyric acid


potassium salt of indole-3-butyric acid


α-naphthalene acetic acid







MS medium

medium of Murashige and Skoog (1962).


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  1. Chen, Z.H., Evans, D.A.: General techniques of tissue culture in perennial crops.—In: Chen, Z., Evans, D.A., Sharp, W.R., Ammirato, P.V., Sondahl, M.R. (ed.): Handbook of Plant Cell Culture. Vol. 6. Pp. 22–61. McGraw-Hill Publishing Company, New York 1990.Google Scholar
  2. Ghosh, B., Sen, S.: Somatic embryos inAsparagus cooperi Baker.—Curr. Sci.58: 256–257, 1989.Google Scholar
  3. Ghosh, B., Sen, S.: Plant regeneration through somatic embryogenesis spear callus culture ofAsparagus cooperi Baker.—Plant Cell Rep.9: 667–670, 1991.CrossRefGoogle Scholar
  4. Ghosh, B., Sen, S.: Stable regeneration inAsparagus cooperi Baker as controlled by different factors. —Plant Sci.82: 119–124, 1992.CrossRefGoogle Scholar
  5. Hu, C.Y., Wang, P.J.: Meristem, shoot tip and bud cultures.—In: Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Pp. 177–227. MacMillan, New York 1983.Google Scholar
  6. Hussey, G.: Problems and prospects in thein vitro propagation of herbaceous plants.—In: Withers, L.A., Anderson, P.G. (ed.): Plant Tissue Culture and its Agricultural Application. Pp. 69–84. Butterworths, London 1986.Google Scholar
  7. Kar, D.K., Sen, S.: Content of sapogenin in diploid, tetraploid and hexaploidAsparagus.—Int. J. Crude Drug Res.24: 131–133, 1986.Google Scholar
  8. Murashige, T.: Plant propagation by tissue culture: A practice with urealized potential.— In: Ammirato, P.V., Evans, D.R., Sharp, W.R., Bajaj, Y.P.S. (ed.): Handbook of Plant Cell Culture. Vol. 5. Pp. 3–9. McGraw-Hill Publishing Company, New York 1990.Google Scholar
  9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco cultures.—Physiol. Plant.15: 473–479, 1962.CrossRefGoogle Scholar
  10. Pierik, R.L.: Rejuvenation and micropropagation.— In: Nijkamp, H.J.J., Van Der Plas, L.H.W., Van Artrigk, J. (ed.): Progress in Plant Cellular and Molecular Biology. Pp. 91–101. Kluwer Academic Publishers, Dordrecht 1990.Google Scholar
  11. Reuther, G.:Asparagus.— In: Sharp, W.R., Evans, D.A., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture Vol. 2. Pp. 211–242. MacMillan Publishing Company, New York 1984.Google Scholar

Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • B. Ghosh
    • 1
  • S. Sen
    • 1
  1. 1.Centre of Advanced Study, Department of BotanyUniversity of CalcuttaCalcuttaIndia

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