Biologia Plantarum

, Volume 36, Issue 4, pp 527–534 | Cite as

Micropropagation ofAsparagus cooperi as affected by growth regulators

  • B. Ghosh
  • S. Sen
Orginal Papers

Abstract

For clonal propagation ofAsparagus cooperi, shoot tips and node explants of 7, 20 and 35 d old spear from the region within 10 cm and below 25 cm from the apex were cultured in modified Murashige and Skoog's (1962) medium containing 6-benzylaminopurine (BAP). The required concentration of BAP varied in explants of different ages and types. In shoot tip culture, the rate of shoot multiplication was higher after 40 d than 60 d of culture. The maximum mumber (62–65) of shoots were obtained from shoot tip explants of 20 d old spear in the medium containing 2.0 mg dm−3 of BAP, 80 mg dm−3 of adenine and 0.02 mg dm−3 of α-naphthalene acetic acid after 60 d of culture. From node cultures, high number of shoots were obtained after 30 d. Pretreatment with BAP in liquid medium for 48 h was effective for semirejuvenescence. The individual shoots produced roots in presence of indole-3-butyric acid and also in potassium salt of indole-3-butyric acid, the later being more effective. All regeneratns were cytologically stable.

Abbreviations

BAP

6-benzylaminopurine

IBA

indole-3-butyric acid

KIBA

potassium salt of indole-3-butyric acid

NAA

α-naphthalene acetic acid

Ade

adenine

2ip

N6-(2-isopentenyl)-adenine

Kin

kinetin

MS medium

medium of Murashige and Skoog (1962).

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Copyright information

© Institute of Experimental Botany 1994

Authors and Affiliations

  • B. Ghosh
    • 1
  • S. Sen
    • 1
  1. 1.Centre of Advanced Study, Department of BotanyUniversity of CalcuttaCalcuttaIndia

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