On the use of enzyme adsorption and specific hydrolysis rate to characterize thermal-chemical pretreatment

  • Shao-Shean Wang
  • Alvin O. Converse
Session 1 Thermal, Chemical, and Biological processing

Abstract

The pseudo enzyme adsorption isotherm of the pretreated substrate is used to estimate the pretreatment’s effectiveness in generating accessible adsorption sites. The specific hydrolysis rate (i.e., the hydrolysis rate divided by the adsorbed enzyme) is used as a measure of substrate reactivity. Poplar, subjected to partial acid hydrolysis at 180 and 200 °C in 1% H2SO4 for 9 s, was contacted with GC 123 (aTrichoderma reesei cellulase produced by Genencor, Inc., South San Francisco, CA). Both adsorption capacity and specific reaction rate increase significantly at the higher pretreatment temperature. The absolute rate and the specific hydrolysis rate fall off rapidly with conversion, and the absolute rate, but not the specific rate, exhibits a maximum as enzyme concentration is increased.

Index Entries

Pretreatment enzyme hydrolysis enzyme adsorption 
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Copyright information

© Humana Press Inc. 1992

Authors and Affiliations

  • Shao-Shean Wang
    • 1
  • Alvin O. Converse
    • 1
  1. 1.Thayer School of EngineeringDartmouth CollegeHanover

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