Production of 2,3- butanediol from pretreated corn cob byKlebsiella oxytoca in the presence of fungal cellulase

  • Ningjun Cao
  • Youkun Xia
  • Cheng S. Gong
  • George T. Tsao
Session 2 Biological Research

Abstract

A simple and effective method of treatment of lignocellulosic material was used for the preparation of corn cob for the production of 2,3-butanediol byKlebsiella oxytoca ATCC 8724 in a simultaneous saccharification and fermentation process. During the treatment, lignin, and alkaline extractives were solubilized and separated from cellulose and hemicellulose fractions by dilute ammonia (10%) steeping. Hemicellulose was then hydrolyzed by dilute hydrochloric acid (1%, wJv) hydrolysis at 100°C at atmospheric pressure and separated from cellulose fraction. The remaining solid, with 90% of cellulose, was then used as the substrate. A butanediol concentration of 25 g/L and an ethanol concentration of 7 g/L were produced byK. oxytoca from 80 g/L of corn cob cellulose with a cellulase dosage of 8.5 IFPU/g corn cob cellulose after 72 h of SSF. With only dilute acid hydrolysis, a butanediol production rate of 0.21 g/L/h was obtained that is much lower than the case in which corn cob was treated with ammonia steeping prior to acid hydrolysis. The butanediol production rate for the latter was 0.36 g/L/h.

Index Entries

Ammonia steeping 2,3-butanediol corn cob Klebsiella oxytoca simultaneous saccharification and fermentation (SSF) 

References

  1. 1.
    Chemical Marketing Reporter (July 24,1995).Google Scholar
  2. 2.
    Garg, S. K. and Jain, A. (1995),Biosource Technol. 51, 103–109.CrossRefGoogle Scholar
  3. 3.
    Jansen, N. B. and Tsao, G. T. (1983),Adv. Biochem. Eng. Biotechnol. 27, 85–100.Google Scholar
  4. 4.
    Jansen, N. B., Flickinger, M. C., and Tsao, G. T. (1984),Biotechnol. Bioeng. 26, 362–369.CrossRefGoogle Scholar
  5. 5.
    Laube, V. M., Groleau, D., and Martin, S. M. (1984),Biotechnol. Lett. 6, 257–262.CrossRefGoogle Scholar
  6. 6.
    Yu, E. K. C., Deschatelets, L., and Saddler, J. N. (1984),Biotech. Bioeng. Symp. 14, 341–352.Google Scholar
  7. 7.
    Dale, B. E. and Moreira, M. J. (1982),Biotech. Bioeng. Symp. 12, 31–43.Google Scholar
  8. 8.
    Grethlein, H. E. and Converse, A. O. (1991),Bioresource Technol. 36, 77–82CrossRefGoogle Scholar
  9. 9.
    McMillan, J. D. (1994), inEnzymic Conversion of Biomass for Fuels Production. Himmel, M. E., Baker, J. O., and Overend, R. P. eds., ACS, Washington, DC, pp. 292–324.Google Scholar
  10. 10.
    Yoon, H. H., Wu, Z. W., and Lee, Y. Y. (1995),Appl. Biochem. Biotechnol. 51/52, 5–19.Google Scholar
  11. 11.
    Takagi, M., Abe, S., Suzuki, S., Emert, G. H., and Yata, N. (1977), inBioconversion Symposium Proceedings, IIT, Delhi, pp. 551–571.Google Scholar
  12. 12.
    Wright, J. D., Wyman, C. E., and Grohmann, K. (1988),Appl. Biochem. Biotechnol. 18, 75–90.Google Scholar
  13. 13.
    Sutcliffe, R. and Saddler, J. N. (1986),Biotechnol. Bioeng. Symp. 17, 749–762.Google Scholar
  14. 14.
    Ooshima, H., Burns, D. S., and Converse, A. O. (1990),Biotecnnol. Bioeng. 36, 446–452.CrossRefGoogle Scholar
  15. 15.
    Elander, R. T. and Hsu, T. (1995),Appl. Biochem. Biotechnol. 51/52, 463–478.Google Scholar
  16. 16.
    Philippidis, G. P. and Smith, T. K. (1995),Appl. Biochem. Biotechnol. 51/52, 117–124.Google Scholar
  17. 17.
    Grohmann, K., Torget, R., and Himmel, M. (1985),Biotech. Bioeng. Symp. 15, 59–80.Google Scholar
  18. 18.
    Foley, K. M. (1978), Chemical properties, physical properties and uses of the Andersons' corncob products. The Andersons, pp. 425.Google Scholar
  19. 19.
    Ghosh, S. (1989),Biomass Handbook, Gordon and Breach Science Publishers, New York, NY, pp. 395,Google Scholar
  20. 20.
    Lawford, H. G. and Rousseau, J. D. (1992),Appl. Biochem. Biotechnol. 34/35, 185–204.Google Scholar
  21. 21.
    Lawford, H. G. and Rousseau, J. D. (1993),Appl. Biochem. Biotechnol. 39/40, 301–322.CrossRefGoogle Scholar
  22. 22.
    Frazer, F. R. and McCaskey, T. A. (1991),Enzyme Micro. Technol. 13, 110–115.CrossRefGoogle Scholar
  23. 23.
    Cao, N. J., Krishnan, M. S., Du, J. X., Gong, C. S., Ho, N. W. Y., Chen Z. D., and Tsao, G. T. (1996),Biotechnol Lett. 18, 1013–1018.CrossRefGoogle Scholar
  24. 24.
    Chen, L. F. and Gong, C. S. (1985),J. Food Sci. 50, 226–228.CrossRefGoogle Scholar
  25. 25.
    Burn, D. S., Ooshima, H., and Converse, A. O. (1989),Appl. Biochem. Biotech. 20/21, 79–94.CrossRefGoogle Scholar
  26. 26.
    Saddler, J. N., Mes-Hartree, M., Yu, E. K. C., and Browell, H. H. (1983),Biotechnol. Bioeng. Symp. 13, 225–238.Google Scholar

Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Ningjun Cao
    • 1
  • Youkun Xia
    • 1
  • Cheng S. Gong
    • 1
  • George T. Tsao
    • 1
  1. 1.Laboratory of Renewable Resources EngineeringPurdue UniversityWest Lafayette

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