Applied Biochemistry and Biotechnology

, Volume 24, Issue 1, pp 253–264 | Cite as

Acetobacter cellulose pellicle as a temporary skin substitute

  • J. D. Fontana
  • A. M. De Souza
  • C. K. Fontana
  • I. L. Torriani
  • J. C. Moreschi
  • B. J. Gallotti
  • S. J. De Souza
  • G. P. Narcisco
  • J. A. Bichara
  • L. F. X. Farah
Session 2 Applied Biological Research


A bacterial strain with morphological and biochemical properties close toAcetobacter xylinum has been cultured in nonagitated, inverted sucrose- and yeast water-based medium for the production of thick, smooth, and floating cellulosic pellicles. The cellulose content (>90%, dry weight, depending on the efficiency of water washing) and theβ-d-homopolyglucan nature of these pellicles were assessed by physical, chemical, and enzymatic methods. The apyrogenic bacterial biomass, a minor component of the dried biofilm (BioFill®), is inactivated by ethylene dioxide. Once applied on exudating or bloody tissues, this biofilm displays several advantages as a biological dressing, and hence, it is valuable as a temporary skin substitute in the treatment of skin wounds, such as burns, ulcers, grafts, and as an adjuvant in dermal abrasions.

Index Entries

Acetobacter cellulose pellicles skin substitute biological dressing 


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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • J. D. Fontana
    • 1
  • A. M. De Souza
    • 1
  • C. K. Fontana
    • 2
  • I. L. Torriani
    • 1
  • J. C. Moreschi
    • 3
  • B. J. Gallotti
    • 3
  • S. J. De Souza
    • 3
  • G. P. Narcisco
    • 3
  • J. A. Bichara
    • 3
  • L. F. X. Farah
    • 3
  1. 1.LQBB-Biomass Chemio/Biotechnology Lab, Department of BiochemistryUFPRCuritiba-PRBrazil
  2. 2.LACEN-FSCMRCuritiba-PRBrazil
  3. 3.BioFill Produtos Biotecnologicos S/ACuritiba-PRBrazil

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