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Applied Biochemistry and Biotechnology

, Volume 70, Issue 1, pp 677–686 | Cite as

Production and purification of tartrate dehydrogenase

Role of aqueous two-phase extraction
  • R. Harve
  • R. K. Bajpai
Session 3: Bioprocessing Research

Abstract

Tartrate dehydrogenase (TDH) is a stereospecific intracellular enzyme produced byPseudomonas putida. Several methods for separation of nucleic acids from the proteins in cell homogenate were compared in this study. These methods included precipitation (using streptomycin sulfate, manganous sulfate, and protamine sulfate) and aqueous two-phase extraction. Under optimal conditions of separation, a single-step aqueous two-phase extraction followed by back-extraction of the enzyme from enzyme-rich PEG-phase resulted in77% recovery of enzyme. This compared favorably with 50% enzyme recovery using protamine sulfate treatment. Furthermore, the remaining enzyme activity was accounted in the nucleic acid-rich dextran phase and the spent-PEG phase, suggesting that a multistep extraction process would increase enzyme recovery even more. Under the conditions of aqueous two-phase extraction, the selectivity of proteins over nucleic acids was 30, indicating a high degree of separation of proteins and nucleic acids in this process. The experimental data and their implications are presented.

Index Entries

Nucleic acids proteins bioseparation precipitation Pseudomonas putida 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • R. Harve
    • 1
  • R. K. Bajpai
    • 1
  1. 1.University of Missouri-Columbia, Chemical Engineering DepartmentUniversity of Missouri-ColumbiaColumbia

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