Optical Review

, 14:151

Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter

  • Yoko Hirohara
  • Yoshitaka OKawa
  • Toshifumi Mihashi
  • Tatsuo Yamaguchi
  • Naoki Nakazawa
  • Yasuko Tsuruga
  • Hiroyuki Aoki
  • Naoyuki Maeda
  • Ichiro Uchida
  • Takashi Fujikado
Regular Papers

DOI: 10.1007/BF02919416

Cite this article as:
Hirohara, Y., OKawa, Y., Mihashi, T. et al. Optical Review (2007) 14: 151. doi:10.1007/BF02919416

Abstract

The purpose of this paper was to investigate the feasibility of a newly developed hyperspectral fundus imaging camera with a liquid crystal tunable filter. The intensities of different wavelengths of light transmitted through an artery, vein, and the area surrounding these vessels and reflected out were measured, and the differential spectral absorptions were analyzed. Measurements were made from 16 normal eyes and from two artificial capillaries. The ratios of absorption (ROA) of arteries to veins from 500 to 580 nm (range 1) and from 600 to 720 nm (range 2) were calculated. For all eyes, the ROArange1 was larger than ROArange2. The ROA obtained from the artificial capillary filled with blood saturated with oxygen or nitrogen was similar to that of simulated data of oxy- and deoxyhemoglobin extinction rate. Most ROAs of human eyes were lower than those of the simulated data and the artificial capillaries. Oxygen saturation analysis by hyperspectral fundus imaging of retinal vessels were qualitatively in agreement with thein vitro analysis or simulated values. However, further improvements are necessary to evaluate the oxygen saturation quantitatively in the retinal blood vessels.

Key words

hyperspectral imaging absorption optical density oxygen saturation liquid crystal wavelength tunable filter 

Copyright information

© The Optical Society of Japan 2007

Authors and Affiliations

  • Yoko Hirohara
    • 1
    • 2
  • Yoshitaka OKawa
    • 1
  • Toshifumi Mihashi
    • 1
    • 2
  • Tatsuo Yamaguchi
    • 2
  • Naoki Nakazawa
    • 2
  • Yasuko Tsuruga
    • 2
  • Hiroyuki Aoki
    • 2
  • Naoyuki Maeda
    • 3
  • Ichiro Uchida
    • 4
  • Takashi Fujikado
    • 1
  1. 1.Department of Applied Visual Science, Graduate School of MedicineOsaka UniversityOsakaJapan
  2. 2.Research InstituteTopcon CorporationTokyoJapan
  3. 3.Department of Ophthalmology, Graduate School of MedicineOsaka UniversityOsakaJapan
  4. 4.Department of Anesthesiology, Graduate School of MedicineOsaka UniversityOsakaJapan

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