Applied Biochemistry and Biotechnology

, Volume 39, Issue 1, pp 559–571 | Cite as

Biological production of ethanol from coal synthesis gas

Medium development studies
  • J. R. Phillips
  • K. T. Klasson
  • E. C. Clausen
  • J. L. Gaddy
Session 4 Bioengineering Research


The anaerobic bacteriaClostridium ljungdahlii produces ethanol and acetate from CO, CO2, and H2 in synthesis gas. Early studies with the bacterium showed that relatively high concentrations of ethanol could be produced by lowering the fermentation pH and eliminating yeast extract from the medium in favor of a defined medium. This article presents the results from a medium development study based on the aerobic bacteriumEscherichia coli. The results of continuous-reactor studies in a continuously stirred tank reactor (CSTR) with and without cell recycle are shown to demonstrate the utility of this improved medium.

Index Entries

C. ljungdahlii ethanol acetate synthesis gas carbon monoxide hydrogen 


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  1. 1.
    Coffin, J. M. (1984),Energy Progress 4, 134–137.Google Scholar
  2. 2.
    Dashekvicz, M. P. and Uffen, R. L. (1979),J. Syst. Bacteriol. 29, 145–148.CrossRefGoogle Scholar
  3. 3.
    Klasson, K. T., Lundbäck, L. M. O., Clausen, E. C., and Gaddy, J. L. (1993),J. Biotechnol. submitted for publication.Google Scholar
  4. 4.
    Lorowitz, W. H. and Bryant, M. P. (1984),Appl. Environ. Microbiol. 47, 961–964.Google Scholar
  5. 5.
    Genthner, B. R. S., and Bryant, M. P. (1982),Appl. Environ. Microbiol. 43, 70–74.Google Scholar
  6. 6.
    Grethlein, A. J., Worden, R. M., Jain, M. K., and Datta, R. (1990),Appl. Biochem. Biotechnol. 24/25, 875–884.Google Scholar
  7. 7.
    Barik, S., Prieto, S., Harrison, S. B., Clausen, E. C., and Gaddy, J. L. (1988),Appl. Biochem. Biotechnol. 18, 363–378.CrossRefGoogle Scholar
  8. 8.
    Vega, J. L., Prieto, S., Elmore, B. B., Clausen, E. C., and Gaddy, J. L. (1989),Appl. Biochem. Biotechnol. 20/21, 781–797.Google Scholar
  9. 9.
    Clausen, E. C., Ackerson, M. D., and Gaddy, J. L. (1991), Biological Production of Ethanol from Coal, Quarterly Report 2-4-91, June–September, Contract No. DE-AC22-89PC89876.Google Scholar
  10. 10.
    Hungate, R. E. (1969), inMethods in Microbiology Vol. 5b, Novic, J. R. and Ribbons, D. W. eds., Academic, New York, pp. 117–132.Google Scholar
  11. 11.
    Ljungdahl, L. G. and Wiegel, J. (1986), inManual of Industrial Microbiology and Biotechnology, Demain, A. L. and Solomon, N. A., eds., American Society for Microbiology, pp. 84–96.Google Scholar
  12. 12.
    McInerney, M. J., Bryant, M. P., and Pfennig, N. (1979),Arch. Microbiol. 122, 129–135.CrossRefGoogle Scholar
  13. 13.
    Genthner, B. R. S., Davis, C. L., and Bryant, M. P. (1981),Appl. Environ. Microbiol. 42, 12–19.Google Scholar
  14. 14.
    Bailey, J. E., and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd ed., McGraw-Hill, New York, p. 28.Google Scholar

Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • J. R. Phillips
    • 1
  • K. T. Klasson
    • 2
  • E. C. Clausen
    • 1
  • J. L. Gaddy
    • 1
  1. 1.Department of Chemical EngineeringUniversity of ArkansasFayetteville
  2. 2.Oak Ridge National LaboratoryOak Ridge

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