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Immunologic Research

, 13:163 | Cite as

Intracellular location of inapparently infecting Chlamydia in synovial tissue from patients with Reiter's syndrome

  • Anna M. Beutler
  • Judith A. Whittum-Hudson
  • Ratanavadee Nanagara
  • H. Ralph Schumacher
  • Alan P. Hudson
Article

Abstract

Culture ofChlamydia trachomatis from synovial tissues/fluids from Reiter's syndrome (RS) patients frequently yields negative results. However, we have identified chlamydial, RNA at that site in such patients suggesting that viable organisms may be present. Here we define the cellular location of chlamydia within the synovium via in situ hybridization. Using a chlamydial ribosomal RNA-directed probe, we show that synovial tissue from culture-negative RS patients gives strong hybridization which is often localized to a subsynovial cell layer, rather than to the synovial lining; in some cases, hybridizing cells are dispersed through the synovium. All hybridization signal is located within host cells, indicating that infectious extracellular elementary bodies are rare or absent. These data confirm the extensive intracellular presence of inapparent chlamydia in the synovia of RS patients and provide some insight into the usual culture negativity of synovial tissues for the organism.

Key Words

Chlamydia Reiter's syndrome In situ hybridization Synovia 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Anna M. Beutler
    • 1
    • 2
  • Judith A. Whittum-Hudson
    • 5
    • 3
  • Ratanavadee Nanagara
    • 1
    • 2
  • H. Ralph Schumacher
    • 1
    • 2
  • Alan P. Hudson
    • 2
    • 4
  1. 1.Division of Rheumatology, Department of MedicineUniversity of Pennsylvania School of MedicinePhiladelphia
  2. 2.Arthritis-Immunology CenterDepartment of Veterans Affairs Medical CenterPhiladelphiaUSA
  3. 3.Medical Research ServiceDepartment of Veterans Affairs Medical CenterPhiladelphiaUSA
  4. 4.Department of Microbiology and ImmunologyMedical College of PennsylvaniaPhiladelphia
  5. 5.Wilmer InstituteJohns Hopkins University School of MedicineBaltimoreUSA

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