Composition of the major elements and trace elements of 10 methanogenic bacteria determined by inductively coupled plasma emission spectrometry
The elemental composition of 10 methanogenic species was determined by inductively coupled plasma emission spectrometry and by a C-H-N-analyzer. The 10 species were representative of all three orders of the methanogens and were cultivated under defined conditions. Special emphasis was given toMethanosarcina barkeri, represented by 5 strains and cultivated on various substrates. The following elements with the lowest and highest values in parentheses were determined: C (37–44%, w/w), H (5.5–6.5%), N (9.5–12,8%); Na (0.3–4.0%), K (0.13–5.0%), S (0.56–1.2%), P (0.5–2.8%), Ca (order I: 85–550 ppm; order II: 1000–4500 ppm), Mg (0.09–0.53%), Fe (0.07–0.28%), Ni (65–180 ppm), Co (10–120 ppm). Mo (10–70 ppm), Zn (50–630 ppm), Cu (<10–160 ppm), Mn (<5–25 ppm). The biggest variations were found with respect to N and K, which both seem to have important physiological functions. Although it is unknown whether zinc and copper are essential trace elements for methanogens, all investigated species contained remarkably high zinc contents, whereas copper seemed to be present only in some species.
Index EntriesMethanogenic bacteria, major and trace elements in Methanosarcina, determination of trace and major elements in sodium, in methanogenic bacteria potassium, in methanogenic bacteria magnesium, in methanogenic bacteria iron, in methanogenic bacteria cobalt, in methanogenic bacteria zinc, in methanogenic bacteria manganese, in methanogenic bacteria sulfur, in methanogenic bacteria phosphorus, in methanogenic bacteria nickel, in methanogenic bacteria molybdenum, in methanogenic bacteria copper, in methanogenic bacteria trace elements, determination in methanogenic bacteria elements, determination in methanogenic bacteria plasma emission spectroscopy, of elements in methanogenic bacteria emission spectroscopy, of elements in methanogenic bacteria spectroscopy, of elements in methanogenic bacteria bacteria, determination of elements in ICP, of methanogenic bacteria
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- 2.H. J. M. Bowen,Trace Elements in Biochemistry, Academic Press, New York, 1966.Google Scholar
- 6.D. Herbert, inMicrobial Reaction to Environment (11th Symp. Soc. General Microbiol.), Cambridge University Press, Cambridge, UK, 1961, pp. 391–416.Google Scholar
- 18.R. A. Mah and M. R. Smith, inThe Prokaryotes (M. P. Starr, H. Stolp, H. G. Trüper, A. Balows, and H. G. Schlegel, eds.), Springer-Verlag, Berlin, 1981, pp. 948–977.Google Scholar
- 25.P. Scherer and H. Sahm, inProceedings of the 1st International Symposium on Anaerobic Digestion, Cardiff, 1979, Poster Papers (D. A. Stafford and B. I. Wheatley, eds.), A. D. Scientific Press, Cardiff, 1980, pp. 45–47.Google Scholar
- 29.C. G. T. P. Schnellen, Thesis, Technical University of Delft, The Netherlands, (Rotterdam: De Maastad, publisher), 1947.Google Scholar
- 33.C. H. Suelter, inCRC Handbook of Microbiology, vol. 4, 2nd ed., A. I. Laskin and H. A. Lechevalier, eds., CRC Press, Boca Raton, Florida, 1982, pp. 553–564.Google Scholar
- 35.G. D. Vogels, J. T. Keltjens, T. J. Hutten, and C. van der Drift,Zbl. Bakt. Hyg. I. Abt. Orig. C 3, 258 (1982).Google Scholar
- 40.S. Yamazaki and L. Tsai,Fed. Proc. 39, p. 1698 (Abstr. 490) (1980).Google Scholar