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Irish Journal of Medical Science

, Volume 172, Issue 2, pp 60–62 | Cite as

Comparison of media for optimal recovery ofCandida albicans andCandida glabrata from blood culture

  • JE Moore
  • R McMullan
Original Paper

Abstract

Background Candida spp., mainlyCandida albicans, are frequently responsible for complications in immunocompromised patients. There are limited data comparing recovery efficiency using simple non-selective basal broth media.

Aim To compare several commercially available basal growth media to determine the medium that gave highest yeast proliferation.

Method Eight commercially available non-selective basal broth culture media were evaluated for optimum recovery of clinicalC. albicans andC. glabrata. They included nutrient broth (NB), nutrient broth no. 2 (NB2), Todd-Hewitt (TH) broth, tryptone soya broth (TSB), tryptone soya broth supplemented with yeast extract (0.5% w/v [TSBYE]), brain heart infusion broth supplemented with yeast extract (0.5% w/v [BHIYE]), salt meat broth (SMB) and 0.1% [w/v] peptone saline (PS). Differences in cell density were evaluated by spectrophotometrical analysis.

Results TSBYE>BHIYE>TSB>TH>NB2>NB>SMB>PS for the optimum proliferation of cells in vitro. Either TSBYE or BHIYE broth may be employed as suitable basal broth media for growth ofC. albicans andC. glabrata. NB should be considered the least suitable medium for routine use when others are available.

Conclusion These data may be of value to laboratories setting up simple blood culture systems to detectCandida spp., particularly in developing and underdeveloped countries.

Keywords

Candida Albicans Nutrient Broth Candida Glabrata Brain Heart Infusion Broth Optimal Recovery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer London 2003

Authors and Affiliations

  1. 1.Department of Bacteriology, Northern Ireland Public Health LaboratoryBelfast City HospitalBelfastNorthern Ireland

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