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Virchows Archiv B

, 64:201 | Cite as

Structural analysis of a new highly metastatic cell line PaTu 8902 from a primary human pancreatic adenocarcinoma

  • Hans-P. Elsässer
  • Ursula Lehr
  • Brigitte Agricola
  • Horst F. Kern
Original Articles

Summary

The new cell line PaTu 8902 was established from a human pancreatic grade II adenocarcinoma of ductal origin. In early passages, cultured cells showed a high degree of heterogeneity in terms of their morphology and the number of chromosomes per cell. When transplanted subcutaneously into nude mice, these cells grew as tumors with a similar morphology and differentiation (grade II) to the primary tumor. In contrast, after prolonged cultivation, cells were more homogenous in terms of their morphology and number of chromosomes per cell, and the corresponding nude mouse xenografts were less differentiated (grade III). When cells from late passages were injected intravenously into nude mice, lung metastases occured after 3–4 weeks. In addition, tumor cells were found in the wall of the esophagus and in the pleural cavity, indicating a high metastatic potential for PaTu 8902 cells in nude mice.

Key words

Pancreatic Adenocarcinoma Human Metastasis 

References

  1. Baylor SM, Berg JW (1973) Cross-classification and survival characteristics of 5000 cases of cancer of the pancreas. J Surg Oncol 5:335PubMedCrossRefGoogle Scholar
  2. Cubilla AL, Fitzgerald PJ (1984) Tumours of the exocrine pancreas. In: Atlas of tumour pathology, second series, fasc. 19. Armed Forces Institute of Pathology, WashingtonGoogle Scholar
  3. Elsässer HP, Lehr U, Agricola B, Kern HF (1992) Establishment and characterisation of two cell lines with different grade of differentiation derived from one primary human pancreatic adenocarcinoma. Virchous Arch [B] 61:295Google Scholar
  4. Gordis L, Gold EB (1986) Epidemiology and etiology of pancreatic cancer. In: Go VLW, Gardner JD, Brooks FP, Lebenthal E, DiMagno EP, Scheele GA (eds) The exocrine pancreas. Raven Press, New York, pp 649–674Google Scholar
  5. Grant AG, Duke D, Hermon-Taylor J (1979) Establishment and characterization of primary human pancreatic carcinoma in continuous cell culture and in nude mice. Br J Cancer 39:143PubMedGoogle Scholar
  6. Ito S, Karnovsky MJ (1968) Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J Cell Biol 39:168Google Scholar
  7. Klöppel G (1984) Pancreatic, non-endocrine tumours. In: Klöppel G, Heitz PU (eds) Pancreatic pathology. Churchill Livington, New York, pp 79–113Google Scholar
  8. Klöppel G, Fitzgerald PJ (1986) Pathology of nonendocrine pancreatic tumours. In: Go VLW, Gardner JD, Brooks FP, Lebenthal E, DiMagno EP, Scheele GA (eds) The exocrine pancreas. Raven Press, New York, pp 649–674Google Scholar
  9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227:680PubMedCrossRefGoogle Scholar
  10. McIntyre LJ, Itzkowitz SH, Kim YS (1986) Human pancreatic cancer cell lines. In: Webber MM (ed) In vitro models for cancer research. CRC Press, Boca RatonGoogle Scholar
  11. Mikal S, Campbell AJA (1950) Carcinaoma of the pancreas. Diagnostic and operative criteria based on one hundred consecutive autopsies. Surgery 28:963PubMedGoogle Scholar
  12. Okabe T, Yamaguchi N, Ohsawa N (1983) Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas. Cancer 51:662PubMedCrossRefGoogle Scholar
  13. Pollänen J, Stephens RW, Vaheri A (1991) Directed plasminogen activation at the surface of normal and malignant cells. Adv Cancer Res 57:273PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Hans-P. Elsässer
    • 1
  • Ursula Lehr
    • 1
  • Brigitte Agricola
    • 1
  • Horst F. Kern
    • 1
  1. 1.Department of Cell Biology and Cell PathologyUniversity of MarburgMarburgGermany

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