Advertisement

Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue

  • J. Hugo Cota-Sánchez
  • Kirsten Remarchuk
  • Kumary Ubayasena
Commentary

Abstract

This report summarizes major changes in previously published protocols for DNA extraction to improve the quality of DNA extracted from plants. Here, we highlight the critical modifications in the original protocols. The efficiency of these changes results in high-quality DNA ready to use in a variety of phytogenetically distant plant families, in particular species with mucopolysaccharides. The DNA obtained can be used without further purification in various molecular biology assays, including direct sequencing and AFLP and RAPD (random-amplified polymorphic DNA) analyses. The effectiveness of this method is proven by the amplification and sequencing of PCR products of up to 1 kb with DNA extracted from herbarium tissue ≥60 years old. This versatility is not usually found in DNA extraction protocols. In addition, this method is quick, adaptable to standard laboratories, and most important, safer and more cost-effective.

Key words

CTAB DNA isolation herbarium specimens mucopolysaccharides 

Abbreviations

CsCl

cesium chloride

NaCl

sodium chloride

NaOAc

sodium oxaloacetate

RAPD

random amplified polymorphic DNA

SEVAG

chloroformsisoamyl alcohol

References

  1. Biss P, Freeland J, Silvertown J, McConway K, and Lutman P (2003) Successful amplification of rice chloroplast microsatellites from century-old grass samples from the Park Grass Experiment. Plant Mol Biol Rep 21: 249–257.CrossRefGoogle Scholar
  2. Cheng YJ, Guo WW, Yi HL, Pang XM, and Deng X (2003) An efficient protocol for genomic DNA extraction fromCitrus species. Plant Mol Biol Rep 21: 177a-177g.CrossRefGoogle Scholar
  3. DeCastro O and Mendle B (2004) PCR amplification of Michele Teniere’s historical specimens and facility to utilize an alternative approach to resolve taxonomic problems. Taxon 53: 147–151.CrossRefGoogle Scholar
  4. Drábková L, Kirschner J, and Vlcek C (2002) Comparison of seven DNA extractions in amplification protocols in historical herbarium specimens of Juncaceae. Plant Mol Biol Rep 20: 161–175.CrossRefGoogle Scholar
  5. Fang G, Hammar S, and Grumet R (1992) A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biofeedback 13: 52–54.Google Scholar
  6. Golenberg EM (1999) Isolation, identification, and authentication of DNA sequences derived from fossil material. In: Jones TP and Rowe NP (eds), Fossil Plants and Spores: Modern Techniques, pp 156–160, The Geological Society, London.Google Scholar
  7. Griffith MP and Porter JM (2003) Back to basics: A simple method of DNA extraction for mucilaginous cacti. Bradleya 21: 126–128.Google Scholar
  8. Jankowiak K, Buczkowska K, and Szweykowka-Kulinska S (2005) Successful extraction of DNA from 100-year-old herbarium specimens of the liverwortBazzania trilobata. Taxon 54: 335–336.Google Scholar
  9. Li YX, Su ZX, and Chen F (2002) Rapid extraction of genomic DNA from leaves and bracts of dove tree (Davidia involucrata). Plant Mol Biol Rep 20: 185a-185e.CrossRefGoogle Scholar
  10. Lodhi MA, Ye G, Weeden NF, and Reisch BI (1994) A simple and efficient method for DNA extraction from grapevine cultivars andVitis species. Plant Mol Biol Rep 12: 6–13.CrossRefGoogle Scholar
  11. Murray MG and Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.PubMedCrossRefGoogle Scholar
  12. Paterson AH, Brubaker CL and Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suiPorebski S, Bailey LG, and Baum BR (1997) Modification of a CTAB DNA extraction protocols for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15: 8–15.Google Scholar
  13. Porebski S, Bailey LG, and Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15: 8–15.CrossRefGoogle Scholar
  14. Saghai-Maroof MA, Soliman KM, Jorgensen RA, and Allard RW (1986) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81: 8014–8018.CrossRefGoogle Scholar
  15. Soltis PS and Soltis PE (1993) Ancient DNA: Prospects and limitations. New Zealand J Bot 31: 203–209.Google Scholar
  16. Taberlet P, Gielly L, Pautou G, and Bouvet J (1991) Universal primers for amplification of three non-coding regions of cpDNA. Plant Mol Biol 17: 1105–1109.PubMedCrossRefGoogle Scholar
  17. Taylor JW and Swann EC (1994) DNA from herbarium specimens. In: Hermann B and Hummel S (eds), Ancient DNA, pp 166–181, Springer-Verlag, New York.Google Scholar
  18. Tel-Zur NS, Abbo S, Myslabodksi D, and Mizrahi Y (1999) Modified CTAB procedure for DNA isolation from epiphytic cacti of the generaHylocereus andSelenicereus (Cactaceae). Plant Mol Biol Rep 17: 249–254.CrossRefGoogle Scholar
  19. Wolf PG, Soltis PS, and Soltis DE (1994) Phylogenetic, relationships of Dennstaedtioid ferns: Evidence fromrbcL. Mol Phylogenet Evol 3: 383–392.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • J. Hugo Cota-Sánchez
    • 1
  • Kirsten Remarchuk
    • 1
  • Kumary Ubayasena
    • 1
  1. 1.Biology DepartmentUniversity of SaskatchewanSaskatoonCanada

Personalised recommendations