Carlsberg Research Communications

, Volume 49, Issue 1, pp 105–109 | Cite as

Location of the proanthocyanidins in the barley grain

  • Sten Aastrup
  • Henrik Outtrup
  • Kenneth Erdal


A vanillin-HCl staining has been used to locate the proanthocyanidins in mature barley grains. These flavanoids were found to be concentrated in the seed coat (testa) just outside the aleurone layer in the 7 varieties with yellow coloured grains investigated. The seed coats of 10 proanthocyanidin-free barley mutants belonging to 4 different gene loci did not stain with vanillin-HCl.


Anthocyanogens proanthocyanidin-free barley vanillin test testa nucellus 


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Copyright information

© Carlsberg Laboratory 1984

Authors and Affiliations

  • Sten Aastrup
    • 1
  • Henrik Outtrup
    • 1
  • Kenneth Erdal
    • 1
  1. 1.Department of Brewing ChemistryCarlsberg Research LaboratoryCopenhagen, Valby

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