Indian Journal of Clinical Biochemistry

, Volume 21, Issue 1, pp 106–110 | Cite as

Relationship of plasma homocysteine with lipid profile parameters in ischemic heart disease


The present study was undertaken to explore the relationship of plasma homocysteine with other biochemical parameters in ischemic heart disease. Plasma levels of total homocysteine was measured by HPLC—fluorescence detection with internal standard in 60 ischemic heart disease patients and were compared with 30 age matched normal healthy controls. The significant increase of plasma homocysteine was observed in both myocardial infarction and chronic stable ischemic heart disease patients when compared with the controls. The hyperhomocysteinemia appears be to due to increased body demand of vitamins such as folic acid, vitamin B12, B6, B2 either alone or in combination to regulate normal homocysteine metabolism.


Ischemic heart disease (IHD) High density lipoprotein (HDL) High performance liquid chromatography (HPLC) Homocysteine (Hcy) Total cholesterol (TC) and Triglycerides (TG) 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Graham, I.M., Daly, L.E. and Refsum, H.M. (1997) Plasma homocysteine as risk factor for vascular disease. The European Concerted Action Project. J. Am. Med. Assocn. 277, 1775–1781.CrossRefGoogle Scholar
  2. 2.
    Wald, N.J., Walt, H.C., Law, M.R., Weir, D.G., Mc Partlin, J. and Scott, J.M. (1998) Homocysteine and ischemic heart disease. Arch. Int. Med. 158, 862–867.CrossRefGoogle Scholar
  3. 3.
    Sathia, G. and Lalitha, S. (2000) Homocysteine and cardiovascular disease. Ind. J. Clin. Practice, 11/6, 59–65.Google Scholar
  4. 4.
    Welch, G. and Loscalzo, J. (1998) Homocysteine and atherothrombosis. N. Eng. J. Med. 338/15, 1042–1050.CrossRefGoogle Scholar
  5. 5.
    Chambers, J.C., Obeid, O.A. and Refsum, H. (2000) Plasma homocysteine concentrations and risk of coronary heart disease in UK, Indian Asians and European men. Lancet 355, 523–527.PubMedCrossRefGoogle Scholar
  6. 6.
    Singh, M. (2000) A case of acute myocardial infarction associated with hyperhomocysteinemia. J. Ind. Acad. Clin. Med. 3/3, 317–318.Google Scholar
  7. 7.
    Ueland, P., Refsum, H., Stabler, S., Malinow, M., Andresson, A. and Allen, R. (1993) Total homocysteine in plasma or serum: Methods and clinical applications. Clin. Chem. 39/9, 1764–1779.Google Scholar
  8. 8.
    Schreiner, R., Gobel-Schreiner, B. and Walch, S. (1995) Homocysteine: Biochemie and Klinische Bedeutung. Clinical Laboratory 41, 1007–1011.Google Scholar
  9. 9.
    Silkers, K.A. and Crit, C.R.C. (1997) Estimation of serum total cholesterol by enzymatic method. Rev. Clin. Lab. Sci. 8, 198.Google Scholar
  10. 10.
    Richmond, W. (1973) Estimation of serum HDL-cholesterol. Clin. Chem. 19, 1350.PubMedGoogle Scholar
  11. 11.
    Buccola, G. and David, M. (1973) Estimation of serum triglycerides. Clin. Chem. 19, 476.Google Scholar
  12. 12.
    Mc Neely, M.D. (1987) Folic acid. In: Presce, A.J., Kaplan, L.A., editors, Methods in clinical laboratory, St. Louis: CV Mosby, Page No. 539–542.Google Scholar
  13. 13.
    Chen, J.W., Sperling, M.I. and Heminger, L.A. (1987) Vitamin B12 In: Presce, A.J., Kaplan, L.A., editors, Methods in clinical laboratory, St. Louis: CV Mosby, Page No. 569–573Google Scholar
  14. 14.
    Kei, Satoh (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica. Chimica. Acta. 90, 37–43.CrossRefGoogle Scholar
  15. 15.
    Cholesterol guidelines (2001) A call for more aggressive lipid lowering. Current Medical Scene, 16/3, 1.Google Scholar
  16. 16.
    Israelsson, B., Brattstrom, L.E. and Hultberg, B.E. (1988) Homocysteine and myocardial infarction. Atherosclerosis 71, 227–233.PubMedCrossRefGoogle Scholar
  17. 17.
    De Bree, A., Verschuren, W.M.M., Kromhout, D., Klujtmans, L.A.J. and Blom, H.J. (2002) Homocysteine determinants and the evidence to what extent homocysteine determine the risk of coronary heart disease. Pharmacol. Rev. 54/4, 599–618.CrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2006

Authors and Affiliations

  1. 1.Department of BiochemistryMIMSR Medical CollegeLatur
  2. 2.Department of BiochemistryShri. Bhausaheb Hire Govt. Medical CollegeDhule
  3. 3.Department of MedicineMIMSR Medical CollegeLatur

Personalised recommendations