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Molecular Biotechnology

, Volume 4, Issue 1, pp 25–43 | Cite as

Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE)

  • Jaan Noolandi
  • Chantal Turmel
Protocol

Abstract

The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, hardware requirements, and procedures are given. A variety of gel pictures for known yeast DNA markers are shown.

Index Entries

One-dimensional pulsed-field gel electrophoresis zero-integrated-field electrophoresis DNA preparation DNA manipulation pulse strategy 

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Jaan Noolandi
    • 1
  • Chantal Turmel
    • 1
  1. 1.Xerox Research Centre of CanadaMississaugaCanada

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