Biologia Plantarum

, Volume 31, Issue 4, pp 280–285 | Cite as

Progeny study of tobacco regenerant transformed by agrobacterium rhizogenes

  • M. OndRej
  • M. Hrouda
  • Anna KoneCnÁ
  • J. VlasÁk
Article
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Abstract

The pRi T-DNA markers were followed in the first generation of the progeny ofAgrobacterium tumefaciens strain C58Cl(pRiA4b) transformed tobacco regenerant. Two categories segregating probably in the ratio 15: 1 can be distinguished: those showing a complex of pRi T-DNA transformation markers and those showing no transformation markers. The presence of both TL and TR-DNA was demonstrated by Southern blotting. The most prominent of the transformation markers observedIn vitro was rapid growth of detached plagiotropic roots on media without phytohormones. Most of the progeny plants of the transformant showed irregularities of leaf morphology and inflorescence and flower morphology. The last resulted in sterility in most progeny plants.

Keywords

Hairy Root Root Culture Crown Gall Agrobacterium Rhizogenes Progeny Plant 
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Copyright information

© Academia 1989

Authors and Affiliations

  • M. OndRej
    • 1
  • M. Hrouda
    • 1
  • Anna KoneCnÁ
    • 1
  • J. VlasÁk
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesČeské BudějoviceCzechoslovakia

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