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Isolation of dihydromyricetin and dihydroquercetin from bark of pinus contorta

  • Henrik Outtrup
  • Kjeld Schaumburg
  • Jørgen Øgaard Madsen
Article

Abstract

Bark of shore pine has high contents of the dihydroflavonols dihydromyricetin and dihydroquercetin. A method for isolation of the pure, optically active compounds in gram amounts has been developed, involving Soxhlet extraction of finely ground, dry bark and preparative scale reversed-phase HPLC. Among a number of inexpensive solvents tested ethyl acetate is useful, being reasonably effective in the extraction step and giving a crude extractive that is suitable for HPLC without any pre-treatment. The use of bark from dead trees, which have been lying in the open for a couple of years, is advantageous because of the lower content of resinous material. 270 MHz1H- and13C-NMR and electron impact mass spectra are presented.

Keywords

Pinus contorta Loud shore pine (+)-dihydromyricetin (+)-dihydroquercetin 1H- 13C NMR mass spectrometry large-scale extraction 

Abbreviations

DiHM

dihydromyricetin

DiHQ

dihydroquercetin

HPLC

high performance liquid chromatography

MS

mass spectrometry

NMR

nuclear magnetic resonance

TMS

tetramethylsilane

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Copyright information

© Carlsberg Laboratory 1985

Authors and Affiliations

  • Henrik Outtrup
    • 1
  • Kjeld Schaumburg
    • 2
  • Jørgen Øgaard Madsen
    • 3
  1. 1.Department of Brewing ChemistryCarlsberg Research CenterCopenhagen Valby
  2. 2.Department of Chemical Physics, The H.C. Ørsted InstituteUniversity of CopenhagenCopenhagen Ø
  3. 3.Department of Organic ChemistryThe Technical University of DenmarkLyngby

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