A non-destructive screening method for proanthocyanidin-free barley mutants

  • Henriette Kristensen
  • Sten Aastrup


Barley grains are embedded in a block of plastic clay with the Carlsberg Seed Fixation System, abraded to expose a small sector of endosperm and surrounding testa. Staining of the proanthocyanidins in the testa is carried out by immersion into a solution of vanillin in 4.5 N−HCl. Kernels lacking proanthocyanidins are selected, washed and grown to mature plants. Using sodium azide mutagenesis 10 proanthocyanidin-free mutants with anthocyanin pigmentation in the vegetative parts of the plants were obtained among 78,450 M3-kernels amounting to a frequency of 0.013%.


Catechin leucocyanidin anthocyanin vanillin-HCl colour reaction 

Copyright information

© Carlsberg Laboratory 1986

Authors and Affiliations

  • Henriette Kristensen
    • 1
  • Sten Aastrup
    • 2
  1. 1.Department of PhysiologyCarlsberg LaboratoryCopenhagen Valby
  2. 2.Department of Brewing ChemistryCarlsberg Research LaboratoryCopenhagen Valby

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