Insulin-like growth factor-1 gene cloning and protein expression in bovine trabecular meshwork tissue and cells

  • Cao Yang
  • Wei Houren
  • Da Banghong
  • Pfaffl Michael
  • Li Zhongyu
Article
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Summary

Whether cultured bovine trabecular meshwork cells and trabecular tissue ex vivo express insulin-like growth factor-I (IGF-I) messenger RNA (mRNA) and protein was investigated. Total RNA of cultured bovine trabecular meshwork cells as well as trabecular meshwork tissue freshly excised from bovine eyes was extracted, and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect IGF-I mRNA. RT-PCR product was verified by sequencing. Immunohistochemical stain was used to detect IGF-I protein. The results showed that a single PCR amplified product was obtained, and the sequence was homologous to the known sequence. IGF-I immunostain was positive in the cytoplasm of trabecular meshwork cells. It was concluded that trabecular meshwork cells produce IGF-I and contribute to the presence of IGF-I in trabecular meshwork microenvironment as well as aqueous humor. Trabecular meshwork cells were affected by IGF-I not only through paracrine, but also autocrine action. Whether abnormal down-regulations in IGF-I production may contribute to the pathogenesis of primary open-angle glaucoma and the possibility of promoting the autocrine action of IGF-I by trabecular meshwork cells to treat the diesease is worth further investigation.

Key words

trabecular meshwork insulin-like growth factor-I reverse transcription-polymerase chain reaction immunohistochemistry primary open-angle glaucoma 
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Copyright information

© Springer 2002

Authors and Affiliations

  • Cao Yang
    • 1
  • Wei Houren
    • 1
  • Da Banghong
    • 1
  • Pfaffl Michael
    • 2
  • Li Zhongyu
    • 3
  1. 1.Department of Ophthalmology, Xiehe Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan
  2. 2.Institute of PhysiologyTechnical University of MunichFreising-WeihenstephanGermany
  3. 3.Department of Anatomy, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

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