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Biologia Plantarum

, 27:154 | Cite as

Tryptophan aminotransferase and tryptophan dehydrogenase activities in some cell compartments of spinach leaves: The effect of calcium ions on tryptophan dehydrogenase

  • Květa Vacková
  • Archana Mehta
  • M. Kutáček
Original Papers

Abstract

The content of spinach-leaf cells was compartmented by differential centrifugation. Three fractions were obtained,i.e. chloroplasts, pellet of remaining organelles sedimenting at 97 000g and cytosol. Enzyme activities of L-tryptophan aminotransferase (TAT) as well as L-tryptophan dehydrogenase (TDH) were demonstrated in all cell fractions. The highest activities of both enzymes were found in the pellet of organelles followed by the enzyme activities in the chloroplasts. The cytosol had the lowest enzyme activities. Chloroplasts are characterized by a relatively higher TDH activity, organelles sedimenting at 97 000g were marked by a relatively higher TAT activity. In all fractions both pyridine nucleotide coenzymes catalyzed the TDH activity. Ca2+ in a concentration of 0.8 minol l−1 increased markedly the TDH activity in both directions of its activity.

Keywords

Auxin Synthesis Tryptophan Aminotransferase Indoleacetaldehyde Phosphoramidothioates Pyridine Nucleotide Coenzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

2-(N-morpholino) ethanesulfonic acid

MES

Na2-ethylenediaminotetraacetate dihydrate

Na2EDTA.2 H2O

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Copyright information

© Academia 1985

Authors and Affiliations

  • Květa Vacková
    • 1
  • Archana Mehta
    • 1
  • M. Kutáček
    • 1
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesPraha 6Czechoslovakia

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