Chinese Science Bulletin

, 49:452

Allergenicity assay of allergen fromDermatophagoides farinae in transgenic tobacco

  • Mingjuan Tang
  • Ye Shen
  • Yuanlei Hu
  • Lei Cao
  • Ting Ni
  • Hongyu Zhang
  • Zhongping Lin
Articles

DOI: 10.1007/BF02900963

Cite this article as:
Tang, M., Shen, Y., Hu, Y. et al. Chin.Sci.Bull. (2004) 49: 452. doi:10.1007/BF02900963
  • 27 Downloads

Abstract

Derf2 gene for one of mite allergens inDermatophagoides farinae has been cloned and expressed under regulation of 35S promoter in transgenic tobacco. The transcriptional analysis showed that this mite complete gene structure in genomic sequence could be spliced at prediction site. Allergenicity assay with immunological sera indicated that the extracts from the transgenic tobacco gave obvious positive IgE binding reaction with specific serum pool. This work would be of potential use in allergenicity assessment of genetically modified food.

Keywords

Dermatophagoides farinae intron splicing Nicotiana tabacum allergenicity 

Copyright information

© Science in China Press 2004

Authors and Affiliations

  • Mingjuan Tang
    • 1
    • 2
  • Ye Shen
    • 1
  • Yuanlei Hu
    • 1
  • Lei Cao
    • 1
  • Ting Ni
    • 1
  • Hongyu Zhang
    • 3
  • Zhongping Lin
    • 1
  1. 1.National Key Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life SciencesPeking UniversityBeijingChina
  2. 2.Institute of Medicinal Plant, Peking Union Medical CollegeChinese Academy Medical ScienceBeijingChina
  3. 3.Department of Allergy, Peking Union Medical College HospitalChinese Academy Medical ScienceBeijingChina

Personalised recommendations