Advertisement

Chinese Science Bulletin

, 46:563 | Cite as

Genetic transformation of calli from maize and regeneration of herbicide-resistant plantlets

  • Guosheng Li
  • Aifang Yang
  • Juren ZhangEmail author
  • Yuping Bi
  • Lei Shan
Notes

Abstract

The herbicide-resistant geneals ofArabidopsis thaliana has been transferred into embryonic calli of maize by microprojectile bombardment. We have obtained chlorsulfuron-resistant calli and regenerated plantlets through selection by herbicide chlorsulfuron. The results of PCR analysis and Southern blotting indicate that geneals has been transferred into some plantlets. The test of spraying chlorsulfuron indicated that the transgenic plantlets had favorable herbicide-resistant trait. The purpose of the research was to obtain chlorsulfuron-resistant transgenic maize and hope that this kind of high efficient herbicide could be widely used in rotation soil of wheat and maize. In addition, through spraying herbicide, we could eliminate the hybrid plants and thereby increase the purity of F1 seeds.

Keywords

maize genetic transformation als chlorsulfuron 

References

  1. 1.
    Haughn, G. W., Somermille, C., Sulfonylurea-resistant mutants ofArabidopsis thaliana, Mol. Gen. Genet., 1986, 204: 430.CrossRefGoogle Scholar
  2. 2.
    Muzar, B. J., Chui, C. F., Smith, J. K., Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides, Plant Physiol., 1987, 85: 1110.CrossRefGoogle Scholar
  3. 3.
    Haughn, G. W., Muzar, B. J., Smith, J., Transformation with a mutantArabidopsis acetolactate synthase gene render tobacco to sulfonylurea herbicides, Mol. Gen. Genet., 1988, 211: 266.CrossRefGoogle Scholar
  4. 4.
    Li, S. R., Zhang, J. R., Chen, H. M., Study on induction of embryogenic callus and plantlet regeneration in maize, Journal of Shandong University (in Chinese), 1990, 25(1): 116.Google Scholar
  5. 5.
    Wang, X. F., Yang, A. F., Zhang, J. R., Genetic transformation of protoplasts fromPeucedanum terebinthaceum and regeneration of herbicide-resistant plantlets, Acta Botanica Sinica (in Chinese), 1999, 41(7): 706.Google Scholar
  6. 6.
    Brettschneider, R., Becker, D., Lörz, H., Efficient transformation of scutellar tissue of immature maize embryos, Ther. Appl. Genet., 1997, 94: 737.CrossRefGoogle Scholar
  7. 7.
    Gordon-Kamm, W. J., Spencer, T. M., Mangano, M. L. et al., Transformation of maize cells and regeneration of fertile transgenic plants, Plant Cell, 1990, 2: 603.PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press 2001

Authors and Affiliations

  • Guosheng Li
    • 1
  • Aifang Yang
    • 1
  • Juren Zhang
    • 1
    Email author
  • Yuping Bi
    • 2
  • Lei Shan
    • 2
  1. 1.School of Life ScienceShandong UniversityJinanChina
  2. 2.Biotechnique Research CenterShandong Agriculture Science InstituteJinanChina

Personalised recommendations