Chinese Science Bulletin

, 46:563 | Cite as

Genetic transformation of calli from maize and regeneration of herbicide-resistant plantlets

  • Guosheng Li
  • Aifang Yang
  • Juren ZhangEmail author
  • Yuping Bi
  • Lei Shan


The herbicide-resistant geneals ofArabidopsis thaliana has been transferred into embryonic calli of maize by microprojectile bombardment. We have obtained chlorsulfuron-resistant calli and regenerated plantlets through selection by herbicide chlorsulfuron. The results of PCR analysis and Southern blotting indicate that geneals has been transferred into some plantlets. The test of spraying chlorsulfuron indicated that the transgenic plantlets had favorable herbicide-resistant trait. The purpose of the research was to obtain chlorsulfuron-resistant transgenic maize and hope that this kind of high efficient herbicide could be widely used in rotation soil of wheat and maize. In addition, through spraying herbicide, we could eliminate the hybrid plants and thereby increase the purity of F1 seeds.


maize genetic transformation als chlorsulfuron 


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Copyright information

© Science in China Press 2001

Authors and Affiliations

  • Guosheng Li
    • 1
  • Aifang Yang
    • 1
  • Juren Zhang
    • 1
    Email author
  • Yuping Bi
    • 2
  • Lei Shan
    • 2
  1. 1.School of Life ScienceShandong UniversityJinanChina
  2. 2.Biotechnique Research CenterShandong Agriculture Science InstituteJinanChina

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