Abundant dendritic cells express HLA-DR in pleomorphic adenomas
- 23 Downloads
Most pleomorphic adenomas were found to contain abundant dendritic cells (DC) with major histo-compatibility complex (MHC) class II (HLA-DR) expression. Their immunohistochemical staining features were suggestive of dendritic histiocytic cells. Extensive phenotypic characterization by two-colour immunofluorescence staining for various cell markers was performed. The DC expressed both HLA class I and II determinants, vimentin, S-100 protein, and various monocyte-related markers (10G11, 3D10, 7G5 or CD11a, 8C2) but were negative for leucocyte common antigen (CD45), Leu-6 (CD1), and the myelomonocytic L1 antigen. Characterization of HLA-DR positive DC isolated by an immunomagnetic bead method confirmed the immunohistochemical staining pattern that corresponds to the phenotype of interdigitating cells. Morphological and immunological implications of the abundant presence of these cells in pleomorphic adenomas are discussed.
Key wordsSalivary gland Pleomorphic adenoma Dendritic cells Immunohistochemistry HLA-DR
Unable to display preview. Download preview PDF.
- Brandtzaeg P (1974) Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with cold ethanol-fixation technique. Immunology 26:1101–1114Google Scholar
- Mason DY (1985) Immunohistochemical labeling of monoclonal antibodies by the APAAP immunoalkaline phosphate technique. In: Bullock GR, Petrusz P (eds) Immunocytochemistry, vol 3. Academic Press, London, pp 25–42Google Scholar
- Murphy GF, Bhau AK, Sato S, Mihm MC, Harrist TJ (1981) A new immunologie marker for human Langerhans cells. N Engl J Med 304:781–792Google Scholar
- Oxholm PO, Manthorpe R, Oxholm A, Schiödt M (1985) Brief report. Langerhans cells in labial minor salivary glands in primary Sjögren’s syndrome. Acta Pathol Microbiol Immunol Scand Sect A 93:105–107Google Scholar
- Raphael M, Debre P, Charron DJ (1984) Reduced Ia-positive Langerhans’ cells in AIDS (letter). N Engl J Med 311:858Google Scholar
- Vaines K, Brandtzaeg P (1985) Retardation of immunfluorescence fading during microscopy. J Histochem Cytochem 83:381–389Google Scholar
- Watamabe S, Nakajima T, Shimosato Y, Shimamura K, Sakuma H (1983) T-zone histiocytes with S100 protein. Development and distribution in human fetuses. Acta Pathol Jpn 33:15–22Google Scholar