Virchows Archiv B

, Volume 59, Issue 1, pp 195–203 | Cite as

Abundant dendritic cells express HLA-DR in pleomorphic adenomas

  • Per Stanley Thrane
  • Ludvig Magne Sollid
  • Per Brandtzaeg


Most pleomorphic adenomas were found to contain abundant dendritic cells (DC) with major histo-compatibility complex (MHC) class II (HLA-DR) expression. Their immunohistochemical staining features were suggestive of dendritic histiocytic cells. Extensive phenotypic characterization by two-colour immunofluorescence staining for various cell markers was performed. The DC expressed both HLA class I and II determinants, vimentin, S-100 protein, and various monocyte-related markers (10G11, 3D10, 7G5 or CD11a, 8C2) but were negative for leucocyte common antigen (CD45), Leu-6 (CD1), and the myelomonocytic L1 antigen. Characterization of HLA-DR positive DC isolated by an immunomagnetic bead method confirmed the immunohistochemical staining pattern that corresponds to the phenotype of interdigitating cells. Morphological and immunological implications of the abundant presence of these cells in pleomorphic adenomas are discussed.

Key words

Salivary gland Pleomorphic adenoma Dendritic cells Immunohistochemistry HLA-DR 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Per Stanley Thrane
    • 1
    • 2
  • Ludvig Magne Sollid
    • 1
  • Per Brandtzaeg
    • 1
  1. 1.Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, Medical FacultyThe National HospitalRikshospitaletNorway
  2. 2.Department of Oral Surgery and Oral Medicine, Dental FacultyUniversity of OsloOsloNorway

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