Mouse epidermal cell renewal after topical treatment with different concentrations of the epidermal peptide pyroGlu-Glu-Asp-Ser-GlyOH
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Earlier work has shown that epidermal cells contain a peptide, pyroGlu-Glu-Asp-Ser-GlyOH, that induces a moderate but long-lasting inhibition of epidermal cell proliferation when given at low (picomol) doses ip in vivo and in vitro. In the present study, the epidermal pentapeptide was applied topically to the back skin of hairless mice at different concentrations and in a water-miscible cream. A single topical application of either high (0.25% wt/wt) or low (0.004% or 0.02% wt/ wt) doses of the pentapeptide was followed by oscillations in epidermal DNA synthesis and G2-M cell flux (mitotic rate). In general, epidermal cell proliferation was inhibited during the first 10-day period after treatment with the two lower doses, while the highest concentration of pentapeptide (0.25%) stimulated epidermal cell proliferation. In spite of the effects on epidermal cell proliferation the size of the epidermal cell population in the treated area (number of nucleated cells and epidermal thickness) showed no corresponding alterations. The results could imply that the epidermal pentapeptide modifies epidermal cell proliferation and terminal differentiation in such a way that the two are balance with each other.
Key wordsEpidermis Growth regulation Peptide Inhibition
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- Christensen HN (1987) Why we might as well forget pyroglutamate as an amino acid. TIPS 9:430Google Scholar
- Eigsti OJ, Dustin Pjr (1955) Colchicine in Agriculture, Medicine, Biology, and Chemistry. The Iowa College Press, AmesGoogle Scholar
- Elgjo K, Clausen OPF (1983) Proliferation-dependent effect of skin extracts (chalone) on mouse epidermal cell flux at the G1-S, S-G2, and G2 -M transitions. Virchows Arch [B] 42:143–151Google Scholar
- Elgjo K, Reichelt KL, Edminson P, Moen E (1986a) Endogenous peptides in epidermal growth control. Serono Symp Publ Raven Press 34:259–266Google Scholar
- Gardner MLG (1985) Production of pharmacologically active peptides from foods in the gut. In: Hunter JO, Alun Jones V (eds) Food and the Gut. Bailliere-Tindall, London, pp 121–134Google Scholar
- Iversen OH, Evensen A (1962) Experimental skin carcinogenesis in mice. Acta Pathol Microbiol Scand [Suppl] 156Google Scholar
- Nome O (1975) Tissue specificity of the epidermal chalones. Virchows Arch [B] 19:1–25Google Scholar
- Paukovits WR, Elgjo K, Laerum OD (1990) Pentapeptide growth inhibitors. In: Sporn MB, Roberts AB (eds) Peptide growth factors and their receptors. Handbook of experimental pharmacology, vol 95 II. Springer, Berlin Heidelberg New York Tokyo, pp 268–295Google Scholar
- Tustin A (1955) Feedback. In: Automatic control. Simon and Schuster, New York, pp 10–32Google Scholar