Virchows Archiv B

, 63:325 | Cite as

Neuroendocrine neoplasms of the lung are not associated with point mutations at codon 12 of the Ki-ras gene

  • Stephan N. Wagner
  • Rupert Müller
  • Joachim Boehm
  • Barbara Pütz
  • Peter H. Wünsch
  • Heinz Höfler


The most prominent abnormality ofras proto-oncogenes in human lung tumours has involved point mutations at codon 12 of the Ki-ras gene. We have analysed 35 tumour samples of neuroendocrine lung neoplasms (ten carcinoid tumours, ten well-differentiated neuroendocrine carcinomas, and 15 intermediate/small cell neuroendocrine carcinomas) for a point mutation at this site. For this purpose, formalin-fixed and paraffin-embedded tissue sections were microdissected to remove non-tumorous areas. DNA in the remaining tumour tissue was amplified in vitro by the polymerase chain reaction (PCR) and double-stranded PCR products were subjected to sequence analysis. Neither point mutations at codon 12 nor additional structural alterations at codons 1–32 were detected in the Ki-ras gene. Our results suggest that point mutations at codon 12 of the Ki-ras gene do not seem to be involved in the pathogenesis of pulmonary neuroendocrine neoplasms.

Key words

Lung Neuroendocrine neoplasms Ki-ras Point mutation Sequencing 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Stephan N. Wagner
    • 1
  • Rupert Müller
    • 2
  • Joachim Boehm
    • 2
  • Barbara Pütz
    • 2
  • Peter H. Wünsch
    • 3
  • Heinz Höfler
    • 1
    • 2
  1. 1.Institute of PathologyGSF-Forschungszentrum für Umwelt und GesundheitNeuherberg-MunichGermany
  2. 2.Institute of Pathology, School of MedicineTechnische Universität MünchenMunich 80Germany
  3. 3.Institute of PathologySchool of Medicine NürnbergNürnberg 90Germany

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