Virchows Archiv B

, Volume 54, Issue 1, pp 252–255 | Cite as

Reduction in phosphoenolpyruvate carboxykinase in rat liver parenchymal cells following experimentally induced cholestasis

  • Cornelis J. F. Van Noorden
  • Ed A. Döpp
  • Christine D. Dijkstra
  • Daniël C. Aronson
  • Wouter H. Lamers
  • André De Graaf
  • Wilma M. Frederiks
Short Communications

Summary

The effect of experimentally induced cholestasis on the amount of phosphoenolpyruvate carboxykinase (PEPCK) was studied immunohistochemically in rat liver parenchyma. In control liver, the enzyme was mainly localized periportally and, although the enzyme content was much reduced, this distribution pattern was maintained up to 2 weeks after ligation of the common bile duct. At 4 and 8 weeks after ligation the enzyme content in parenchymal cells remained low, but became distributed homogeneously throughout the liver parenchyma. This suggests that after bile duct ligation, gluconeogenesis from lactate is impaired. This may well be the cause of the adaptive changes to enhance the glycogenolytic capacity of parenchymal cells to maintain as far as possible a constant blood glucose level.

Key words

Cholestasis Phosphoenolpyruvate carboxykinase Carbohydrate metabolism Glu-cose metabolism Liver Immunohistochemistry 

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References

  1. Andersen B, Nath A, Jungermann K (1982) Heterogeneous distribution of phosphoenolpyruvate carboxykinase in rat liver parenchyma, isolated, and cultured hepatocytes. Eur J Cell Biol 28:47–53PubMedGoogle Scholar
  2. Aronson DC, De Haan J, James J, Bosch KS, Ketel AG, Houtkooper JM, Heijmans HSA (1988) Quantitative aspects of the parenchyma-stroma relationship in experimentally induced cholestasis. Liver (in press)Google Scholar
  3. Butcher RG (1972) Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry. Histochemie 32:171–190PubMedCrossRefGoogle Scholar
  4. Dijkstra CD, Döpp EA, Vogels IMC, Van Noorden CJF (1987) Macrophages and dendritic cells in antigen-induced arthritis: an immunohistochemical study using cryostat sections of the whole knee joint of rat. Scand J Immunol 26:513–523PubMedCrossRefGoogle Scholar
  5. Graham RC, Karnovsky ML (1966) The early stages of absorption of injected peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302PubMedGoogle Scholar
  6. Guder WG, Schmidt U, Funk B, Weiss J, Pürschel S (1976) Liver cell heterogeneity. The distribution of pyruvate kinase and phosphoenolpyruvate carboxykinase in the liver lobule of fed and starved rats. Hoppe-Seyler’s Z Physiol Chem 357:1793–1800PubMedGoogle Scholar
  7. Hardie G (1982) Kinetic behaviour of membrane-bound, irreversible enzyme systems: experimental and theoretical considerations. J Histochem Cytochem 30:1083–1085PubMedGoogle Scholar
  8. James J, Bosch KS, Zuyderhoudt FMJ, Houtkooper JM, Van Gool J (1986) Histophotometric estimation of volume density of collagen as an indication of fibrosis in rat liver. Histochemistry 85:129–133PubMedCrossRefGoogle Scholar
  9. Johnstone JMS, Lee EG (1976) A quantitative assessment of the structural changes in the rat’s liver following obstruction of the common bile duct. Br J Exp Pathol 57:85–94PubMedGoogle Scholar
  10. Lamers WH, Van Roon M, Mooren PG, De Graaf A, Charles R (1985) Amino acid environment determines expression of carbamoylphosphate synthetase and phosphoenolpyruvate carboxykinase in embryonic rat hepatocytes. In Vitro 21:606–611Google Scholar
  11. Lee EG, Ross BD, Haines JR (1972) The effect of experimental bile-duct obstruction on critical biosynthetic functions of the liver. Br J Surg 59:564–568PubMedCrossRefGoogle Scholar
  12. Nibbering PH, Leijh PCJ, Van Furth R (1985) A cytophotometric method to quantitate the binding of monoclonal antibodies to individual cells. J Histochem Cytochem 33:453–459PubMedGoogle Scholar
  13. Nibbering PH, Marijnen JGJ, Raap AK, Leijh PCJ, Van Furth R (1986) Quantitative study of enzyme immunocytochemical reactions performed with enzyme conjugates immobilized on nitrocellulose. Histochemistry 84:538–543PubMedCrossRefGoogle Scholar
  14. Probst I, Jungermann K (1983) The glucagon-insulin antagonism and glucagon-dexamethasone synergism in the induction of phosphoenolpyruvate carboxykinase in cultured rat hepatocytes. Hoppe-Seyler’s Z Physiol Chem 364:1739–1764PubMedGoogle Scholar
  15. Sasaki K, Cripe PT, Koch SR, Andreone TL, Petersen DD, Beate EG, Granner D (1984) Multihormonal regulation of phosphoenolpyruvate carboxykinase gene transcription. J Biol Chem 259:15242–15251PubMedGoogle Scholar
  16. Schölmerich J, Becher MS, Baumgartner U, Gerok W (1985) Loss of glucagon control of gluconeogenesis in liver cells from rats with bile duct obstruction. Biochem Biophys Res Commun 126:1146–1153PubMedCrossRefGoogle Scholar
  17. Van Noorden CJF, Butcher RG (1986) The out-of-range error in microdensitometry. Histochem J 18:397–398PubMedCrossRefGoogle Scholar
  18. Van Noorden CJF, Frederiks WM, Aronson DC, Marx F, Bosch K, Jonges GN, Vogels IMC, James J (1987) Changes in the acinar distribution of some enzymes involved in carbohydrate metabolism in rat liver parenchyma after experimentally induced cholestasis. Virchows Arch [Cell Pathol] 52:501–511CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Cornelis J. F. Van Noorden
    • 1
  • Ed A. Döpp
    • 2
  • Christine D. Dijkstra
    • 2
  • Daniël C. Aronson
    • 3
  • Wouter H. Lamers
    • 4
  • André De Graaf
    • 4
  • Wilma M. Frederiks
    • 1
  1. 1.Laboratory of Histology and Cell BiologyUniversity of Amsterdam, Academic Medical CentreAmsterdam
  2. 2.Department of HistologyMedical Faculty, Free UniversityAmsterdam
  3. 3.Department of Pediatrics, University of Amsterdam and Department of SurgeryUniversity of LeidenNetherlands
  4. 4.Department of Anatomy and EmbryologyUniversity of AmsterdamAmsterdamThe Netherlands

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