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Chinese Science Bulletin

, Volume 43, Issue 9, pp 784–787 | Cite as

New technology to examine and improve the purity of hybrid rice with herbicide resistant gene

  • Danian Huang
  • Jingyang Li
  • Shanqing Zhang
  • Rui Xue
  • Wei Yang
  • Zhihua Hua
  • Xiaobo Xie
  • Xiaoling Wang
Bulletin

Abstract

The herbicide resistant bar gene has been widely used as selectable marker genes in the study on plant genetic transformation. Owing to the integration of the gene into rice genome, transgenic rice was resistant to the herbicide Basta. Therefore, selection of transgenic plant md genetic analysis became easier. In the studies, bar gene was introduced as a genetic marker gene into ria restore lie variety of two-line or three-line. Combined with conventional breeding method, the good herbicide resistant individual plant was derived as the new restorc line for hybrid combination. After sprayed with Basta at seeding phasc. the real hybrid rice that hnd herbicide resistance could grow normally, while the fnke hybrid rice and weeds, having no bar gene, were killed by herbicide. The method described above helped rapidly to examine and improve the purity of hybrid rice.

Keywords

herbicide resistant bar gene transgenic hybrid rice purity of hybrid rice 

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References

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    Huang, D. N., Zhu, B., Yang, W.et al., Introduction of cecropin B gene into rice (Oryza Sativa L.) by particle bombardment and analysis of trensgenic plants,Science in China, Ser. C, 1997, 39(6): 652.Google Scholar
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Copyright information

© Science in China Press 1998

Authors and Affiliations

  • Danian Huang
    • 1
  • Jingyang Li
    • 1
  • Shanqing Zhang
    • 1
  • Rui Xue
    • 1
  • Wei Yang
    • 1
  • Zhihua Hua
    • 1
  • Xiaobo Xie
    • 1
  • Xiaoling Wang
    • 1
  1. 1.China National Rice Research InstituteHangzhouChina

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