Abstract
The copy number of genes encoding 5S ribosomal RNA has been found to be constant in Petunia hybrida plants regenerated from protoplast and leaf disc-derived callus cultures. However, in one somaclone a heritable change in the length of the major 5S rDNA repeat has arisen. Despite the constant copy number of 5S rRNA genes, that of the 18S–25S rRNA genes is found to very by at least ten-fold. The relevance of these findings to ribosomal RNA gene variability and to somaclonal variation is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, S., Lewis-Smith, A. C., Smith, S. M.: Methylation of ribosomal RNA genes inPetunia hybrida plants, callus cultures and regenerated shoots. - Plant Cell Rep.8:554–557, 1990.
Breiman, A., Rotem-Abarbanell, D., D., Karp, A., Shaskin, H.: Heritable somaclonal variation in wild barley (Hordeum spontaneum). - Theor. appl. Genet.74: 104–112, 1987.
Brettell, R. I. S., Pallotta, M. A., Gustafson, J. P., Appels, R.: Variation at theNor loci in triticale derived from tissue culture. - Theor. appl. Genet.71: 637–643, 1986.
Feinberg, A. P., Vogelstein, B.: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activities. - Anal. Biochem.137: 266–267, 1984.
Gerlach, W. L., Bedbrook, J. R.: Cloning and characterisation of ribosomal RNA genes from wheat and barley. - Nucl. Acids Res.7:1869–1885, 1979.
Gerlach, W. L., Dyer, T. A.: Sequence organisation of the repeating units in the nucleus of wheat which contains 5S rRNA genes. - Nucl. Acids Res.8: 4851–4865, 1980.
Landsmann, J., Uhrig, H.: Somaclonal variation in Solanum tuberosum detected at the molecular level. - Theor. appl. Genet.71: 500–505, 1985.
Lee, M., Phillips, R. L.: The chromosomal basis of somaclonal variation. - Annu. Rev. Plant Physiol. mol. Biol.39: 413–437, 1988.
Lewis-Smith, A. C., Chamberlain, M., Smith, S. M.: Genetic and chromosomal variation inPetunia hybrida plants regenerated from protoplast and callus cultures. - Biol. Plant.4: 247–255, 1990.
Pental, D. Hamill, J. D., Pirrie, A., Cocking, E.: Somatic hybridisation ofNicotiana tabacum and Petuniahybrida. - Mol. gen. Genet.202: 342–347, 1986.
Rode, A., Hartmann, C., Benslimane, A., Picard, E., Quetier, F.: Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L., cv. Cesar). - Theor. appl. Genet.74: 31–37, 1987.
Rogers, S. O., Bendich, A. J.: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. -Plant mol. Biol.9: 509–520, 1987.
Scoles, G. J., Gill, B. S., Xin, Z.-Y., Clarke, B. C., Mc Intyre, C. L., Chapman, C., Appels, R.: Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae. - Plant Syst. Evol.160:105–112, 1988.
Shepherd, A. L., Anderson, S., Smith, S. M.: Species-specific repeated DNA sequences fromPetunia-Plant Sci.67: 57–62, 1990.
Waldron, J., Dunsmuir, P., Bedbrook, J.: Characterisation of the rDNA repeats in the MichellPetunia genome. - Plant mol. Biol.2: 57–65, 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anderson, S., Lewis-Smith, A.C., Chamberlain, M. et al. Variation in organisation and copy number of ribosomal RNA genes inPetunia hybrida somaclones. Biol Plant 33, 206–210 (1991). https://doi.org/10.1007/BF02897884
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897884